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Literature summary for 3.5.1.11 extracted from

  • Cai, G.; Zhu, S.; Yang, S.; Zhao, G.; Jiang, W.
    Cloning, overexpression, and characterization of a novel thermostable penicillin G acylase from Achromobacter xylosoxidans: probing the molecular basis for its high thermostability (2004), Appl. Environ. Microbiol., 70, 2764-2770.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Achromobacter xylosoxidans

Inhibitors

Inhibitors Comment Organism Structure
phenylmethylsulfonyl fluoride strong Achromobacter xylosoxidans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0089
-
penicillin G pH 8.0, 37°C Achromobacter xylosoxidans
0.027
-
6-nitro-3-[(phenylacetyl)amino]benzoic acid pH 8.0, 37°C Achromobacter xylosoxidans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
x * 27000, alpha-subunit, + x * 62400, beta-subunit, SDS-PAGE Achromobacter xylosoxidans
62400
-
x * 27000, alpha-subunit, + x * 62400, beta-subunit, SDS-PAGE Achromobacter xylosoxidans

Organism

Organism UniProt Comment Textmining
Achromobacter xylosoxidans Q83YY8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-nitro-3-[(phenylacetyl)amino]benzoic acid + H2O
-
Achromobacter xylosoxidans 3-amino-6-nitrobenzoic acid + phenylacetic acid
-
?
penicillin G + H2O
-
Achromobacter xylosoxidans 6-aminopenicillanate + phenylacetic acid
-
?

Subunits

Subunits Comment Organism
? x * 27000, alpha-subunit, + x * 62400, beta-subunit, SDS-PAGE Achromobacter xylosoxidans

Synonyms

Synonyms Comment Organism
PGA650
-
Achromobacter xylosoxidans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Achromobacter xylosoxidans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
half-life of inactivation 55 min Achromobacter xylosoxidans
60
-
half-life of inactivation 8 min Achromobacter xylosoxidans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1
-
penicillin G pH 8.0, 37°C Achromobacter xylosoxidans
68.8
-
6-nitro-3-[(phenylacetyl)amino]benzoic acid pH 8.0, 37°C Achromobacter xylosoxidans
72.7
-
penicillin G pH 8.0, 37°C Achromobacter xylosoxidans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Achromobacter xylosoxidans