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Literature summary for 3.5.1.11 extracted from

  • Galunsky, B.; Lummer, K.; Kasche, V.
    Comparative study of substrate- and stereospecificity of penicillin G amidases from different sources and hybrid isoenzymes (2000), Monatsh. Chem., 131, 623-632.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
production of penicillin amidase hybrid I and hybrid II in Escherichia coli. Hybrid A contains A-chain from K. citrophila and B-chain from Escherichia coli. Hybrid II contains A-chain from Escherichia coli and B-chain from Kluyvera citrophila Escherichia coli
production of penicillin amidase hybrid I and hybrid II in Escherichia coli. Hybrid A contains A-chain from Kluyvera citrophila and B-chain from Escherichia coli. Hybrid II contains A-chain from Escherichia coli and B-chain from Kluyvera citrophila Kluyvera cryocrescens

Organism

Organism UniProt Comment Textmining
Alcaligenes faecalis
-
-
-
Escherichia coli
-
-
-
Kluyvera cryocrescens
-
-
-
Rhizobium viscosum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R) -2-(4-hydroxyphenyl)glycine amide + H2O
-
Escherichia coli (R) -2-(4-hydroxyphenyl)glycine + hydroxylamine
-
?
(R)-2-phenylglycine amide + H2O
-
Alcaligenes faecalis (R)-2-phenylglycine + hydroxylamine
-
?
(R)-2-phenylglycine amide + H2O
-
Escherichia coli (R)-2-phenylglycine + hydroxylamine
-
?
(R)-2-phenylglycine amide + H2O
-
Rhizobium viscosum (R)-2-phenylglycine + hydroxylamine
-
?
(R)-2-phenylglycine amide + H2O
-
Kluyvera cryocrescens (R)-2-phenylglycine + hydroxylamine
-
?
(R)-2-phenylglycine methyl ester + H2O
-
Alcaligenes faecalis (R)-2-phenylglycine + methanol
-
?
(R)-2-phenylglycine methyl ester + H2O
-
Escherichia coli (R)-2-phenylglycine + methanol
-
?
(R)-2-phenylglycine methyl ester + H2O
-
Rhizobium viscosum (R)-2-phenylglycine + methanol
-
?
(R)-2-phenylglycine methyl ester + H2O
-
Kluyvera cryocrescens (R)-2-phenylglycine + methanol
-
?
2-nitro-5-[(phenylacetyl)amino]benzoic acid + H2O
-
Alcaligenes faecalis phenylacetic acid + 5-amino-2-nitrobenzoic acid
-
?
2-nitro-5-[(phenylacetyl)amino]benzoic acid + H2O
-
Escherichia coli phenylacetic acid + 5-amino-2-nitrobenzoic acid
-
?
2-nitro-5-[(phenylacetyl)amino]benzoic acid + H2O
-
Rhizobium viscosum phenylacetic acid + 5-amino-2-nitrobenzoic acid
-
?
2-nitro-5-[(phenylacetyl)amino]benzoic acid + H2O
-
Kluyvera cryocrescens phenylacetic acid + 5-amino-2-nitrobenzoic acid
-
?
5-[[(2R)-2-amino-2-phenylethanoyl]amino]-2-nitrobenzoic acid + H2O
-
Escherichia coli 5-amino-2-nitrobenzoic acid + (R)-2-phenylglycine
-
?
ampicillin + H2O
-
Escherichia coli (R)-2-phenylglycine + 6-aminopenicillanic acid
-
?
N-(phenylacetyl)glycine + H2O
-
Escherichia coli phenylacetic acid + glycine
-
?
N-phenylacetyl-Asp + H2O
-
Escherichia coli phenylacetic acid + Asp
-
?
N-phenylacetyl-Asp-Phe methyl ester + H2O
-
Escherichia coli phenylacetic acid + Asp-Phe methyl ester
-
?
N-phenylacetyl-Glu + H2O
-
Escherichia coli phenylacetic acid + Glu
-
?
N-phenylacetyl-Leu + H2O
-
Escherichia coli phenylacetic acid + Leu
-
?
N-phenylacetyl-Phe + H2O
-
Escherichia coli phenylacetic acid + Phe
-
?
N2-phenylacetyl-2'-deoxyguanosine + H2O
-
Escherichia coli phenylacetic acid + 2'-deoxyguanosine
-
?
N6-phenylacetyl-2'-deoxyadenosine + H2O
-
Escherichia coli phenylacetic acid + 2'-deoxyadenosine
-
?
penicillin G + H2O
-
Alcaligenes faecalis 6-aminopenicillanic acid + phenylacetic acid
-
?
penicillin G + H2O
-
Escherichia coli 6-aminopenicillanic acid + phenylacetic acid
-
?
penicillin G + H2O
-
Rhizobium viscosum 6-aminopenicillanic acid + phenylacetic acid
-
?
penicillin G + H2O
-
Kluyvera cryocrescens 6-aminopenicillanic acid + phenylacetic acid
-
?

Synonyms

Synonyms Comment Organism
PA
-
Alcaligenes faecalis
PA
-
Escherichia coli
PA
-
Rhizobium viscosum
PA
-
Kluyvera cryocrescens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2
-
(R) -2-(4-hydroxyphenyl)glycine amide
-
Escherichia coli
12000
-
N-phenylacetyl-Glu
-
Escherichia coli

pI Value

Organism Comment pI Value Maximum pI Value
Escherichia coli
-
-
6.7