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Literature summary for 3.5.1.108 extracted from

  • Hyland, S.A.; Eveland, S.S.; Anderson, M.S.
    Cloning, expression, and purification of UDP-3-O-acyl-GlcNAc deacetylase from Pseudomonas aeruginosa: a metalloamidase of the lipid A biosynthesis pathway (1997), J. Bacteriol., 179, 2029-2037.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
subcloned the Pseudomonas aeruginosa lpxC gene into the T7-based expression vector pET11a Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
EDTA 0.002 mM, complete inactivation, reactivated by the addition of excess heavy metal Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
additional information metalloenzyme Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33435
-
x * 33435, calculated from sequence Pseudomonas aeruginosa
38600
-
x * 38600, SDS-PAGE Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine + H2O Pseudomonas aeruginosa a committed step of lipopolysaccharide biosynthesis UDP-3-O-((R)-3-hydroxymyristoyl)-D-glucosamine + acetate
-
?
UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine + H2O Escherichia coli committed step of lipopolysaccharide biosynthesis UDP-3-O-((R)-3-hydroxymyristoyl)-D-glucosamine + acetate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Pseudomonas aeruginosa P47205
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli
-
Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.33
-
-
Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine + H2O
-
Escherichia coli UDP-3-O-((R)-3-hydroxymyristoyl)-D-glucosamine + acetate
-
?
UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine + H2O
-
Pseudomonas aeruginosa UDP-3-O-((R)-3-hydroxymyristoyl)-D-glucosamine + acetate
-
?
UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine + H2O a committed step of lipopolysaccharide biosynthesis Pseudomonas aeruginosa UDP-3-O-((R)-3-hydroxymyristoyl)-D-glucosamine + acetate
-
?
UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine + H2O committed step of lipopolysaccharide biosynthesis Escherichia coli UDP-3-O-((R)-3-hydroxymyristoyl)-D-glucosamine + acetate
-
?

Subunits

Subunits Comment Organism
? x * 33435, calculated from sequence Pseudomonas aeruginosa
? x * 38600, SDS-PAGE Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
LpxC protein
-
Pseudomonas aeruginosa
UDP-3-O-acyl-GlcNAc deacetylase
-
Escherichia coli
UDP-3-O-acyl-GlcNAc deacetylase
-
Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Escherichia coli
8.5
-
-
Pseudomonas aeruginosa

pH Range

pH Minimum pH Maximum Comment Organism
5 7 pH 5.0: about 45% of maximal activity, pH 7.0: about 60% of maximal activity Escherichia coli
7 9 pH 7.0: about 55% of maximal activity, pH 9.0: about 75% of maximal activity Pseudomonas aeruginosa