Cloned (Comment) | Organism |
---|---|
subcloned the Pseudomonas aeruginosa lpxC gene into the T7-based expression vector pET11a | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 0.002 mM, complete inactivation, reactivated by the addition of excess heavy metal | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | metalloenzyme | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33435 | - |
x * 33435, calculated from sequence | Pseudomonas aeruginosa |
38600 | - |
x * 38600, SDS-PAGE | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine + H2O | Pseudomonas aeruginosa | a committed step of lipopolysaccharide biosynthesis | UDP-3-O-((R)-3-hydroxymyristoyl)-D-glucosamine + acetate | - |
? | |
UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine + H2O | Escherichia coli | committed step of lipopolysaccharide biosynthesis | UDP-3-O-((R)-3-hydroxymyristoyl)-D-glucosamine + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Pseudomonas aeruginosa | P47205 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.33 | - |
- |
Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine + H2O | - |
Escherichia coli | UDP-3-O-((R)-3-hydroxymyristoyl)-D-glucosamine + acetate | - |
? | |
UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine + H2O | - |
Pseudomonas aeruginosa | UDP-3-O-((R)-3-hydroxymyristoyl)-D-glucosamine + acetate | - |
? | |
UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine + H2O | a committed step of lipopolysaccharide biosynthesis | Pseudomonas aeruginosa | UDP-3-O-((R)-3-hydroxymyristoyl)-D-glucosamine + acetate | - |
? | |
UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine + H2O | committed step of lipopolysaccharide biosynthesis | Escherichia coli | UDP-3-O-((R)-3-hydroxymyristoyl)-D-glucosamine + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33435, calculated from sequence | Pseudomonas aeruginosa |
? | x * 38600, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
LpxC protein | - |
Pseudomonas aeruginosa |
UDP-3-O-acyl-GlcNAc deacetylase | - |
Escherichia coli |
UDP-3-O-acyl-GlcNAc deacetylase | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Escherichia coli |
8.5 | - |
- |
Pseudomonas aeruginosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | pH 5.0: about 45% of maximal activity, pH 7.0: about 60% of maximal activity | Escherichia coli |
7 | 9 | pH 7.0: about 55% of maximal activity, pH 9.0: about 75% of maximal activity | Pseudomonas aeruginosa |