Activating Compound | Comment | Organism | Structure |
---|---|---|---|
HslU | protease HslV is activated by the ATPase HslU. Mutations in hslV that disrupt the interaction with the C termini of HslU invariably lead to inactive enzyme | Haemophilus influenzae |
Protein Variants | Comment | Organism |
---|---|---|
DELTA83-92 | hydrolysis of casein, SulA-MBP or benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin is less than 20% of the activity of the wild-type enzyme | Haemophilus influenzae |
DELTA86-91 | hydrolysis of casein, SulA-MBP or benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin is unchanged | Haemophilus influenzae |
K28A | hydrolysis of casein, SulA-MBP or benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin is less than 20% of the activity of the wild-type enzyme | Haemophilus influenzae |
R35A | hydrolysis of casein, SulA-MBP or benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin is less than 20% of the activity of the wild-type enzyme | Haemophilus influenzae |
R86D | hydrolysis of casein, SulA-MBP or benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin is less than 20% of the activity of the wild-type enzyme | Haemophilus influenzae |
R89A/K90A | hydrolysis of casein, SulA-MBP or benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin is less than 20% of the activity of the wild-type enzyme | Haemophilus influenzae |
R89D | hydrolysis of casein, SulA-MBP or benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin is 40-60% of the activity of the wild-type enzyme | Haemophilus influenzae |
R89D/K90E | hydrolysis of casein and SulA-MBP is less than 20% of the activity of the wild-type enzyme, hydrolysis of benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin is higher than that of the wild-type enzyme | Haemophilus influenzae |
V112A | hydrolysis of casein, SulA-MBP or benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin is less than 20% of the activity of the wild-type enzyme | Haemophilus influenzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemophilus influenzae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin + H2O | - |
Haemophilus influenzae | benzyloxycarbonyl-GGL + 7-amino-4-methylcoumarin | - |
? | |
casein + H2O | - |
Haemophilus influenzae | ? | - |
? |