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Literature summary for 3.4.24.75 extracted from

  • Cohen, D.N.; Sham, Y.Y.; Haugstad, G.D.; Xiang, Y.; Rossmann, M.G.; Anderson, D.L.; Popham, D.L.
    Shared catalysis in virus entry and bacterial cell wall depolymerization (2009), J. Mol. Biol., 387, 607-618.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.75

Crystallization (Commentary)

Crystallization (Comment) Organism
Computational modeling of the gene product 13-muropeptide complex for analysing the orientation of the scissile bond Salasvirus phi29

Protein Variants

Protein Variants Comment Organism
D195A mutant of gene product 13, reduced general protein folding and Zn2+ binding (circular dichroism and particle-induced X-ray emission spectroscopy) Salasvirus phi29
D195C mutant of gene product 13, general protein folding and Zn2+ binding are maintained (circular dichroism and particle-induced X-ray emission spectroscopy) Salasvirus phi29

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ general protein folding and Zn2+ binding are maintained in the D195C mutant but reduced in the D195A mutant Salasvirus phi29

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18400
-
 N-terminal domain of gene product 13, 159 amino acids, calculated mass Salasvirus phi29
22300
-
 C-terminal domain of gene product 13, 202 amino acids, calculated mass Salasvirus phi29
40900
-
 gp13 wild-type, 365 amino acids, calculated mass Salasvirus phi29

Organism

Organism UniProt Comment Textmining
Salasvirus phi29
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 gene product 13 is a protein at the tail tip of the Bacillus subtilis bacteriophage phi29 with D,D-endopeptidase functionality Salasvirus phi29

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information following pseudoinfection of sacculi with bacteriophage phi29 and gene protein 13 respectively: release of muropeptides (predominantly p7) into the supernatant, a modest decrease in the cross-linked muropeptide p21 Salasvirus phi29 ?
-
 ?
additional information gene product 13 does not cause cleavage of pentaglycine Salasvirus phi29 ?
-
 ?

Subunits

Subunits Comment Organism
heterodimer C-terminal and N-terminal domain Salasvirus phi29

Synonyms

Synonyms Comment Organism
lysostaphin analysis of Bacillus subtilis bacteriophage phi29 gene product 13's lysostaphin activity Salasvirus phi29