Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.75 extracted from

  • Bardelang, P.; Vankemmelbeke, M.; Zhang, Y.; Jarvis, H.; Antoniadou, E.; Rochette, S.; Thomas, N.R.; Penfold, C.N.; James, R.
    Design of a polypeptide fluorescence resonance energy transfer substrate that facilitates study of the antimicrobial protease lysostaphin (2009), Biochem. J., 418, 615-624.
    View publication on PubMed
Search for more literature for 3.4.24.75

Cloned(Commentary)

Cloned (Comment) Organism
recominant expression of His-tagged lysostaphin by Escherichia coli BL21 (DE3) pEA3 Staphylococcus simulans

Protein Variants

Protein Variants Comment Organism
D37A no endopeptidase activity Staphylococcus simulans
H114A no endopeptidase activity Staphylococcus simulans
H116A no endopeptidase activity Staphylococcus simulans
H33A no endopeptidase activity Staphylococcus simulans
H83A no endopeptidase activity Staphylococcus simulans
T107A reduced endopeptidase activity Staphylococcus simulans
Y81A reduced endopeptidase activity Staphylococcus simulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16260
-
 ESI-TOF MS, endopeptidase domain (residues 1-140) Staphylococcus simulans
28010
-
 ESI-TOF MS, residues 247-493 Staphylococcus simulans

Organism

Organism UniProt Comment Textmining
Staphylococcus simulans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
zinc metal-chelate chromatography, cation-exchange chromatography Staphylococcus simulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
MV11 + H2O developing a fluorescence resonance energy transfer substrate based on MV11 Staphylococcus simulans ?
-
 ?

Synonyms

Synonyms Comment Organism
lysostaphin
-
 Staphylococcus simulans