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Literature summary for 3.4.24.75 extracted from

  • Turner, M.S.; Waldherr, F.; Loessner, M.J.; Giffard, P.M.
    Antimicrobial activity of lysostaphin and a Listeria monocytogenes bacteriophage endolysin produced and secreted by lactic acid bacteria (2006), Syst. Appl. Microbiol., 30, 58-67.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli and Lactococcus lactis Lactiplantibacillus plantarum
expressed in Escherichia coli and Lactococcus lactis Limosilactobacillus fermentum
expressed in Escherichia coli and Lactococcus lactis Lacticaseibacillus rhamnosus
expression of the functional mature enzyme fused to secretion signals of the Sep protein from Lactobacillus fermentum BR11, to direct the enzyme secretion from recombinant cells, in Lactobacillus fermentum strain BR11, Lactobacillus rhamnosus strain GG, Lactobacillus plantarum ATCC 14917 and Lactococcus lactis strain MG1363, method development and production levels, overview Staphylococcus simulans

Organism

Organism UniProt Comment Textmining
Lacticaseibacillus rhamnosus
-
-
-
Lacticaseibacillus rhamnosus GG
-
-
-
Lactiplantibacillus plantarum
-
strain ATCC 14917
-
Limosilactobacillus fermentum
-
-
-
Limosilactobacillus fermentum BR11
-
-
-
Staphylococcus simulans
-
biovar staphylolyticus, strain NRRL B-2628
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the preprolysostaphin is proteolytically processed to the mature enzyme Staphylococcus simulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is a glycylglycine endopeptidase Staphylococcus simulans ?
-
?
Peptidoglycan + H2O
-
Lactiplantibacillus plantarum Fragments of peptidoglycan
-
?
Peptidoglycan + H2O
-
Limosilactobacillus fermentum Fragments of peptidoglycan
-
?
Peptidoglycan + H2O
-
Lacticaseibacillus rhamnosus Fragments of peptidoglycan
-
?
Peptidoglycan + H2O
-
Limosilactobacillus fermentum BR11 Fragments of peptidoglycan
-
?
Peptidoglycan + H2O
-
Lacticaseibacillus rhamnosus GG Fragments of peptidoglycan
-
?

Synonyms

Synonyms Comment Organism
Glycylglycine endopeptidase
-
Lactiplantibacillus plantarum
Glycylglycine endopeptidase
-
Limosilactobacillus fermentum
Glycylglycine endopeptidase
-
Lacticaseibacillus rhamnosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Lactiplantibacillus plantarum
7.5
-
-
Limosilactobacillus fermentum
7.5
-
-
Lacticaseibacillus rhamnosus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5
-
7% of activity is observed at pH 5.5 Lacticaseibacillus rhamnosus
5.5
-
7% of activity is observed at pH of 5.5 Lactiplantibacillus plantarum
5.5
-
7% of activity is observed at pH of 5.5 Limosilactobacillus fermentum