Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21(DE3) | Staphylococcus simulans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26900 | - |
calculated from the plot of mobility versus log molecular weight of standards | Staphylococcus simulans |
27000 | - |
SDS-PAGE, mature enzyme | Staphylococcus simulans |
27200 | - |
theoretically estimated value | Staphylococcus simulans |
29000 | - |
Sephacryl S-200 gel filtration, His6-fused lysostaphin | Staphylococcus simulans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus simulans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal affinity chromatography on Ni-NTA resin | Staphylococcus simulans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
11900 units per mg, after 12 fold purification, 1 unit is defined as the amount of enzyme required to decrease the OD(A600) of a suspension of Staphylococcus aureus 237 cells by 0.001 at 37°C in 5 min in a 3 ml reaction volume | Staphylococcus simulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Peptidoglycan + H2O | - |
Staphylococcus simulans | Fragments of peptidoglycan | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Glycylglycine endopeptidase | - |
Staphylococcus simulans |
r-lysostaphin | - |
Staphylococcus simulans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | - |
Staphylococcus simulans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 30 | stable for 24 hours at pH 8.0 | Staphylococcus simulans |
40 | - |
stable for 4h with 80% activity | Staphylococcus simulans |
50 | - |
when incubated at temperatures from 25 to 70°C the enzyme is stable up to 50°C, above the activity is rapidly lost | Staphylococcus simulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Staphylococcus simulans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Staphylococcus simulans |