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Literature summary for 3.4.24.75 extracted from

  • Ramadurai, L.; Lockwood, K.J.; Nadakavukaren, M.J.; Jayaswal, R.K.
    Characterization of a chromosomally encoded glycylglycine endopeptidase of Staphylococcus aureus (1999), Microbiology, 145, 801-808.
No PubMed abstract available
Search for more literature for 3.4.24.75

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
ampicillin
-
 Staphylococcus aureus
Ba2+
-
 Staphylococcus aureus
Cu2+
-
 Staphylococcus aureus
diammonium sulfate
-
 Staphylococcus aureus
diethyldicarbonate
-
 Staphylococcus aureus
DTT
-
 Staphylococcus aureus
EDTA
-
 Staphylococcus aureus
Fe2+
-
 Staphylococcus aureus
HgCl2
-
 Staphylococcus aureus
KCl 100 mM Staphylococcus aureus
Mg2+
-
 Staphylococcus aureus
Mn2+
-
 Staphylococcus aureus
NaCl 100 mM Staphylococcus aureus
PMSF
-
 Staphylococcus aureus
Zn2+
-
 Staphylococcus aureus

Metals/Ions

Metals/Ions Comment Organism Structure
Zinc 0.9 mol zinc per mol LytM Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Staphylococcus aureus LytM is expressed during early exponential phase and is overexpressed in an autolysis-defective mutant compared with the parent strain. LytM plays a role in cell growth ?
-
 ?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Staphylococcus aureus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information LytM is expressed during early exponential phase and is overexpressed in an autolysis-defective mutant compared with the parent strain. LytM plays a role in cell growth Staphylococcus aureus ?
-
 ?
Peptidoglycan + H2O
-
 Staphylococcus aureus Fragments of peptidoglycan
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 15 min, stable. Activity is destroyed after 30 min Staphylococcus aureus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 8
-
 Staphylococcus aureus