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Literature summary for 3.4.24.75 extracted from

  • Sugai, M.; Fujiwara, T.; Akiyama, T.; Ohara, M.; Komatsuzawa, H.; Inoue, S.; Suginaka, H.
    Purification and molecular characterization of glycylglycine endopeptidase produced by Staphylococcus capitis EPK1 (1997), J. Bacteriol., 179, 1193-1202.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Staphylococcus capitis

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Staphylococcus capitis
Cu2+
-
Staphylococcus capitis
diethyldicarbonate
-
Staphylococcus capitis
iodoacetate
-
Staphylococcus capitis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Staphylococcus capitis
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Na+ stimulation Staphylococcus capitis
NH4+ stimulation Staphylococcus capitis
Zn2+ metalloprotease, 1 mol of Zn2+ per mol of enzyme, atomic absorption spectroscopy Staphylococcus capitis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35600
-
mature enzyme, calculation from nucleotide sequence Staphylococcus capitis
39310
-
proenzyme, calculation from nucleotide sequence Staphylococcus capitis
48000
-
SDS-PAGE in presence and absence of reducing agents Staphylococcus capitis

Organism

Organism UniProt Comment Textmining
Staphylococcus capitis
-
EPK1
-
Staphylococcus capitis EPK1
-
EPK1
-

Purification (Commentary)

Purification (Comment) Organism
-
Staphylococcus capitis

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Staphylococcus capitis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no hydrolysis of beta-casein Staphylococcus capitis ?
-
?
additional information no hydrolysis of beta-casein Staphylococcus capitis EPK1 ?
-
?
Peptidoglycan + H2O hydrolyis of pentaglycine interpeptide bridges of Staphylococcal peptidoglycan Staphylococcus capitis Fragments of peptidoglycan
-
?
Peptidoglycan + H2O hydrolyis of pentaglycine interpeptide bridges of Staphylococcal peptidoglycan Staphylococcus capitis EPK1 Fragments of peptidoglycan
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9
-
Staphylococcus capitis