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Literature summary for 3.4.24.66 extracted from
- Isolation and some properties of low choriolytic enzyme (LCE), a component of the hatching enzyme of the teleost, Oryzias latipes (1989), J. Biochem., 105, 212-218.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 0.5 mM, choriolytic and caseinolytic activity, 1 mM Zn2+ restores, 5 mM Cu2+ partially restores, not Ca2+ or Mg2+ | Oryzias latipes |  |
| additional information | no inhibition of caseinolytic activity by 1-10 mM IAA, monoiodoacetic acid or diisopropyl fluorophosphate | Oryzias latipes |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Oryzias latipes | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Calcium | 0.00538 mg/mg protein, inductively coupled plasma emission analysis | Oryzias latipes | |
| Magnesium | 0.00164 mg/mg protein, inductively coupled plasma emission analysis | Oryzias latipes | |
| additional information | no cobalt, manganese or iron detected by inductively coupled plasma emission analysis | Oryzias latipes | |
| Zinc | 0.00124 mg/mg protein, inductively coupled plasma emission analysis | Oryzias latipes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid composition | Oryzias latipes |
| 25500 | - |
1 * 25500, Oryzias latipes, SDS-PAGE | Oryzias latipes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Swollen chorion + H2O | Oryzias latipes | i.e. inner layer of chorion that has been swollen by previous action of choriolysin H, EC 3.4.24.67, essential reaction in choriolysis, component of embryo-secreted hatching enzyme that digests egg envelope | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryzias latipes | - |
medaka, teleost fish, orange-red variety | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from hatching liquid | Oryzias latipes |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hatching liquid | i.e. culture medium of blastulae | Oryzias latipes | - |
| hatching liquid | taken immediately after larvae hatched on the 6th day | Oryzias latipes | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Oryzias latipes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide + H2O | hydrolysis at about 8% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide | Oryzias latipes | ? | - |
? | |
| casein + H2O | - |
Oryzias latipes | ? | - |
? | |
| additional information | poor substrates are succinyl-Ala-Pro-Ala 4-methylcoumarin 7-amide, succinyl-Ala-Ala-Pro-Phe 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Leu-Ser-Thr-Arg 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Leu-Thr-Arg 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Val-Leu-Lys 4-methylcoumarin 7-amide, no substrates are intact chorion or Gly-Pro 4-methylcoumarin 7-amide | Oryzias latipes | ? | - |
? | |
| Succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide + H2O | best substrate of peptidyl 4-methylcoumarin 7-amides | Oryzias latipes | ? | - |
? | |
| Swollen chorion + H2O | i.e. partially digested chorion | Oryzias latipes | ? | - |
? | |
| Swollen chorion + H2O | i.e. inner layer of chorion that has been swollen by previous action of choriolysin H, EC 3.4.24.67, essential reaction in choriolysis, component of embryo-secreted hatching enzyme that digests egg envelope | Oryzias latipes | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | 1 * 25500, Oryzias latipes, SDS-PAGE | Oryzias latipes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 9.8 | Oryzias latipes |
| 8.5 | - |
caseinolytic activity | Oryzias latipes |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | about 50% of maximal caseinolytic activity at pH 6.5 and 8.9 | Oryzias latipes |
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