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Literature summary for 3.4.24.56 extracted from

  • Song, E.; Rodgers, D.; Hersh, L.
    A monomeric variant of insulin degrading enzyme (IDE) loses its regulatory properties (2010), PLoS ONE, 5, e9719.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.56

Activating Compound

Activating Compound Comment Organism Structure
ATP
-
 Rattus norvegicus
bradykinin
-
 Rattus norvegicus
dynorphin B-9
-
 Rattus norvegicus
Triphosphate
-
 Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information mutant with a deleted putative dimer interface in the C-terminal region is created, which results in a monomeric variant. Monomeric IDE retains enzymatic activity. Monomeric IDE retains, 25% of the wild type activity substrate (Abz-GGFLRKHGQ-EDDnp). With the peptide substrates beta-endorphin and amyloid beta peptide 1-40, monomeric IDE retains 1 to 0.25% of wild type activity. No activation through bradykinin or dynorphin B-9. Monomeric IDE is not activated by polyphosphates Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0066
-
 Abz-GGFLRKHGQ-EDDnp pH 7.4, temperature not specified in the publication, mutant DELTAC lacking the C-terminal region Rattus norvegicus
0.0215
-
 Abz-GGFLRKHGQ-EDDnp pH 7.4, temperature not specified in the publication, wild-type Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-GGFLRKHGQ-EDDnp + H2O
-
 Rattus norvegicus Abz-GGFLR + KHGQ-EDDnp
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Rattus norvegicus
monomer mutant with a deleted putative dimer interface in the C-terminal region is created, which results in a monomeric variant. Monomeric IDE retains 25% of the wild type activity substrate (Abz-GGFLRKHGQ-EDDnp). With the peptide substrates beta-endorphin and amyloid beta peptide, monomeric IDE retains 1 to 0.25% of wild type activity. No activation through bradykinin or dynorphin B-9. Monomeric IDE is not activated by polyphosphates Rattus norvegicus

Synonyms

Synonyms Comment Organism
IDE
-
 Rattus norvegicus
Insulysin
-
 Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
 Abz-GGFLRKHGQ-EDDnp pH 7.4, temperature not specified in the publication, mutant DELTAC lacking the C-terminal region Rattus norvegicus
0.043
-
 Abz-GGFLRKHGQ-EDDnp pH 7.4, temperature not specified in the publication, wild-type Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Rattus norvegicus