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Literature summary for 3.4.24.56 extracted from

  • Ralat, L.A.; Guo, Q.; Ren, M.; Funke, T.; Dickey, D.M.; Potter, L.R.; Tang, W.J.
    Insulin-degrading enzyme modulates the natriuretic peptide-mediated signaling response (2011), J. Biol. Chem., 286, 4670-4679.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
protein ANP
-
Homo sapiens
protein BNP
-
Homo sapiens
protein CNP
-
Homo sapiens
protein DNP
-
Homo sapiens
urodilatin
-
Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(7-methoxycoumarin-4-yl)acetyl-RPPGFSAFK-2,4-dinitrophenyl + H2O
-
Homo sapiens ?
-
?
protein ANP + H2O
-
Homo sapiens ?
-
?
protein BNP + H2O
-
Homo sapiens ?
-
?
protein CNP + H2O
-
Homo sapiens ?
-
?
protein DNP + H2O
-
Homo sapiens ?
-
?
urodilatin + H2O
-
Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
IDE
-
Homo sapiens
Insulin-degrading enzyme
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
protein DNP pH not specified in the publication, 37°C Homo sapiens
0.2
-
protein BNP pH not specified in the publication, 37°C Homo sapiens
2
-
urodilatin pH not specified in the publication, 37°C Homo sapiens
10
-
protein ANP pH not specified in the publication, 37°C Homo sapiens
20
-
protein CNP pH not specified in the publication, 37°C Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.04
-
pH not specified in the publication, 37°C Homo sapiens protein ANP
0.07
-
pH not specified in the publication, 37°C Homo sapiens protein CNP
0.12
-
pH not specified in the publication, 37°C Homo sapiens protein BNP
0.8
-
pH not specified in the publication, 37°C Homo sapiens urodilatin
1.3
-
pH not specified in the publication, 37°C Homo sapiens protein DNP

General Information

General Information Comment Organism
malfunction reduction of IDE expression diminishes the ability of natriuretic peptides ANP and BNP to stimulate natriuretic peptide receptor NPR-B Homo sapiens
physiological function reducing expression levels of IDE profoundly alters the response of natriuretic peptide receptors (NPR-A and NPR-B) to the stimulation of ANP, BNP, and CNP in cultured cells. IDE rapidly cleaves ANP and CNP, thus inactivating their ability to raise intracellular cGMP. Reduced IDE expression enhances the stimulation of NPR-A and NPR-B by ANP and CNP, respectively. IDE cleavage can lead to hyperactivation of BNP toward NPR-A. Conversely, decreasing IDE expression reduces BNP-mediated signaling Homo sapiens