Literature summary for 3.4.22.65 extracted from

de Halleux, S.; Stura, E.; VanderElst, L.; Carlier, V.; Jacquemin, M.; Saint-Remy, J.
Three-dimensional structure and IgE-binding properties of mature fully active Der p 1, a clinically relevant major allergen (2006), J. Allergy Clin. Immunol., 117, 571-576.

Search for more literature for 3.4.22.65

Application

Application	Comment	Organism
medicine	affects individuals with allergy, resulting in rhinitis, asthma and/or atopic dermatitits	Dermatophagoides pteronyssinus

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Pichia pastoris X-33	Dermatophagoides pteronyssinus

Crystallization (Commentary)

Crystallization (Comment)	Organism
4°C, 45% monomethyl polyethylene glycole 5000 or 2000 in 50 mM sodium acetate (pH 5.5), 1.9 A resolution, typical papain-family fold, exhibits a magnesium binding site	Dermatophagoides pteronyssinus

Protein Variants

Protein Variants	Comment	Organism
N52Q	mutant with disrupted N-glycosylation motif in the prodomain	Dermatophagoides pteronyssinus

Inhibitors

Inhibitors	Comment	Organism	Structure
4-(2-aminoethyl) benzenesulfonyl fluoride	-	Dermatophagoides pteronyssinus
benzamidine	-	Dermatophagoides pteronyssinus
E-64	cysteine protease-specific inhibitor, 0.1 mM, complete inhibition	Dermatophagoides pteronyssinus
phenylmethanesulfonyl fluoride	-	Dermatophagoides pteronyssinus

Organism

Organism	UniProt	Comment	Textmining
Dermatophagoides pteronyssinus	-	-	-
Dermatophagoides pteronyssinus	P08176	-	-

Purification (Commentary)

Purification (Comment)	Organism
dialysis, cationic exchange on SP-Sepharose, recombinant Der p 1 dialysis, benzamidine column, dialysis	Dermatophagoides pteronyssinus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-tert-butoxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O	-	Dermatophagoides pteronyssinus	N-tert-butoxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin	-	?

Synonyms

Synonyms	Comment	Organism
Der p 1	-	Dermatophagoides pteronyssinus
major house dust mite allergen	-	Dermatophagoides pteronyssinus

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Release 2023.1 (January 2023)