Literature summary for 3.4.22.15 extracted from

Adams-Cioaba, M.A.; Krupa, J.C.; Xu, C.; Mort, J.S.; Min, J.
Structural basis for the recognition and cleavage of histone H3 by cathepsin L (2011), Nat. Commun., 2, 197.

Search for more literature for 3.4.22.15

Crystallization (Commentary)

Crystallization (Comment)	Organism
the inactive C25A cathepsin L mutant is cocrystallized with a histone H3 peptide covering residues 19-33 of the human histone H3 tail (QLATKAARKSAPATG), sitting drop vapor diffusion method, using 10% (v/v) isopropanol, 0.1 M sodium acetate trihydrate, pH 4.0, 22% polyethylene glycol 6000. The final crystallization condition for the apo-mC25A crystals is 0.2 M ammonium sulfate, 0.1 M sodium acetate trihydrate, pH 4.6, 30% (w/v) polyethylene glycol 2000 monomethylether	Homo sapiens

Crystallization (Comment)

Organism

the inactive C25A cathepsin L mutant is cocrystallized with a histone H3 peptide covering residues 19-33 of the human histone H3 tail (QLATKAARKSAPATG), sitting drop vapor diffusion method, using 10% (v/v) isopropanol, 0.1 M sodium acetate trihydrate, pH 4.0, 22% polyethylene glycol 6000. The final crystallization condition for the apo-mC25A crystals is 0.2 M ammonium sulfate, 0.1 M sodium acetate trihydrate, pH 4.6, 30% (w/v) polyethylene glycol 2000 monomethylether

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
C25A	inactive	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism
KAPR-acetyl-K-QLATKAARKSAPA	-	Homo sapiens
KAPRKQLAT-acetyl-K-AARKSAPA	-	Homo sapiens
KAPRKQLATKAAR-dimethyl-K-SAPA	-	Homo sapiens
KAPRKQLATKAARKSAPA	-	Homo sapiens
L-trans-epoxysuccinylleucylamido-(4-guanidino)butane	i.e. E-64, complete inhibition at 0.26 mM	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.000037	-	Abz-PRKQLAT-acetyl-K-AARKSAK-Dnp	pH and temperature not specified in the publication	Homo sapiens
0.000063	-	Abz-PRKQLATKAARKSAK-Dnp	pH and temperature not specified in the publication	Homo sapiens
0.000073	-	Abz-PRKQLATKAAR-dimethyl-K-SAK-Dnp	pH and temperature not specified in the publication	Homo sapiens
0.00016	-	Abz-PR-acetyl-K-QLATKAARKSAK-Dnp	pH and temperature not specified in the publication	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
nucleus	-	Homo sapiens	5634	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
histone H3 + H2O	Homo sapiens	-	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P07711	-	-

Purification (Commentary)

Purification (Comment)	Organism
CM Sepharose column chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
Abz-PR-acetyl-K-QLATKAARKSAK-Dnp + H2O	-	Homo sapiens	?	-	?
Abz-PRKQLAT-acetyl-K-AARKSAK-Dnp + H2O	-	Homo sapiens	?	-	?
Abz-PRKQLATKAAR-dimethyl-K-SAK-Dnp + H2O	-	Homo sapiens	?	-	?
Abz-PRKQLATKAARKSAK-Dnp + H2O	-	Homo sapiens	?	-	?
histone H3 + H2O	-	Homo sapiens	?	-	?
L-Leu-L-Arg-7-amido-4-methylcoumarin + H2O	-	Homo sapiens	L-Leu-L-Arg + 7-amino-4-methylcoumarin	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.37	-	Abz-PRKQLAT-acetyl-K-AARKSAK-Dnp	pH and temperature not specified in the publication	Homo sapiens
0.46	-	Abz-PRKQLATKAARKSAK-Dnp	pH and temperature not specified in the publication	Homo sapiens
0.49	-	Abz-PR-acetyl-K-QLATKAARKSAK-Dnp	pH and temperature not specified in the publication	Homo sapiens
0.51	-	Abz-PRKQLATKAAR-dimethyl-K-SAK-Dnp	pH and temperature not specified in the publication	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism
0.0045	-	KAPRKQLATKAARKSAPA	pH and temperature not specified in the publication	Homo sapiens
0.00455	-	KAPRKQLATKAAR-dimethyl-K-SAPA	pH and temperature not specified in the publication	Homo sapiens
0.00502	-	KAPRKQLAT-acetyl-K-AARKSAPA	pH and temperature not specified in the publication	Homo sapiens
0.0107	-	KAPR-acetyl-K-QLATKAARKSAPA	pH and temperature not specified in the publication	Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
2900	-	Abz-PR-acetyl-K-QLATKAARKSAK-Dnp	pH and temperature not specified in the publication	Homo sapiens
5300	-	Abz-PRKQLATKAAR-dimethyl-K-SAK-Dnp	pH and temperature not specified in the publication	Homo sapiens
6100	-	Abz-PRKQLATKAARKSAK-Dnp	pH and temperature not specified in the publication	Homo sapiens
6700	-	Abz-PRKQLAT-acetyl-K-AARKSAK-Dnp	pH and temperature not specified in the publication	Homo sapiens