Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteine | activation with 10 mM cysteine-HCl for 2 h at 37°C | Fasciola hepatica | |
cysteine | activation with 10 mM cysteine-HCl for 2 h at 37°C | Fasciola gigantica |
Cloned (Comment) | Organism |
---|---|
expressed in yeast | Fasciola hepatica |
expressed in yeast | Fasciola gigantica |
Protein Variants | Comment | Organism |
---|---|---|
L69W | isoform CatL5 mutant, compared to the wild type enzyme the mutant has a higher efficiency of cleavage (kcat) against tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin | Fasciola hepatica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Albumin + H2O | Fasciola hepatica | isoform CatL5 shows weak activity against albumin | ? | - |
? | |
Fibronectin + H2O | Fasciola hepatica | cleavage occurs only at pH 5.5 | ? | - |
? | |
Fibronectin + H2O | Fasciola gigantica | cleavage occurs only at pH 5.5 | ? | - |
? | |
Immunoglobulin + H2O | Fasciola hepatica | - |
? | - |
? | |
Immunoglobulin + H2O | Fasciola gigantica | - |
? | - |
? | |
Laminin + H2O | Fasciola hepatica | cleavage occurs only at pH 5.5 | ? | - |
? | |
Laminin + H2O | Fasciola gigantica | cleavage occurs only at pH 5.5 | ? | - |
? | |
additional information | Fasciola hepatica | isoform CatL1G can cleave substrates with proline residues at P2 | ? | - |
? | |
additional information | Fasciola gigantica | isoform CatL1G can cleave substrates with proline residues at P2, isoform CatL1G is unable to digest albumin or hemoglobin, efficiently at either pH 5.5 or 7.0 | ? | - |
? | |
type 1 collagen + H2O | Fasciola hepatica | efficient cleavage of type 1 collagen by isoform CatL5 is limited to the beta-and gamma-chains | ? | - |
? | |
type 1 collagen + H2O | Fasciola gigantica | isoform Catl1G completely digests collagen type 1 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fasciola gigantica | - |
- |
- |
Fasciola hepatica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel-chelating Sepharose column chromatography | Fasciola gigantica |
nickel-chelating Sepharose column chromatography, gel filtration | Fasciola hepatica |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Albumin + H2O | isoform CatL5 shows weak activity against albumin | Fasciola hepatica | ? | - |
? | |
benzoyl-L-Phe-L-Val-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Fasciola hepatica | benzoyl-L-Phe-L-Val-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzoyl-L-Phe-L-Val-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Fasciola gigantica | benzoyl-L-Phe-L-Val-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
D-Val-Leu-Arg-7-amido-4-methylcoumarin + H2O | - |
Fasciola hepatica | D-Val-Leu-Arg + 7-amino-4-methylcoumarin | - |
? | |
D-Val-Leu-Arg-7-amido-4-methylcoumarin + H2O | - |
Fasciola gigantica | D-Val-Leu-Arg + 7-amino-4-methylcoumarin | - |
? | |
Fibronectin + H2O | cleavage occurs only at pH 5.5 | Fasciola hepatica | ? | - |
? | |
Fibronectin + H2O | cleavage occurs only at pH 5.5 | Fasciola gigantica | ? | - |
? | |
Immunoglobulin + H2O | - |
Fasciola hepatica | ? | - |
? | |
Immunoglobulin + H2O | - |
Fasciola gigantica | ? | - |
? | |
L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Fasciola gigantica | L-Phe-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O | preferred substrate for isoform CatL5 | Fasciola hepatica | L-Phe-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
L-Pro-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Fasciola hepatica | L-Pro-L-Phe-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
L-Pro-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Fasciola gigantica | L-Pro-L-Phe-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
Laminin + H2O | cleavage occurs only at pH 5.5 | Fasciola hepatica | ? | - |
? | |
Laminin + H2O | cleavage occurs only at pH 5.5 | Fasciola gigantica | ? | - |
? | |
additional information | isoform CatL1G can cleave substrates with proline residues at P2 | Fasciola hepatica | ? | - |
? | |
additional information | isoform CatL1G can cleave substrates with proline residues at P2, isoform CatL1G is unable to digest albumin or hemoglobin, efficiently at either pH 5.5 or 7.0 | Fasciola gigantica | ? | - |
? | |
tert-butyloxycarbonyl-L-Ala-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Fasciola hepatica | tert-butyloxycarbonyl-L-Ala-Gly-L-Pro-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
tert-butyloxycarbonyl-L-Ala-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Fasciola gigantica | tert-butyloxycarbonyl-L-Ala-Gly-L-Pro-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
tert-butyloxycarbonyl-L-Asp-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Fasciola hepatica | tert-butyloxycarbonyl-L-Asp-L-Pro-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
tert-butyloxycarbonyl-L-Asp-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Fasciola gigantica | tert-butyloxycarbonyl-L-Asp-L-Pro-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
tert-butyloxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin + H2O | - |
Fasciola hepatica | tert-butyloxycarbonyl-L-Val-L-Leu-L-Lys + 7-amino-4-methylcoumarin | - |
? | |
tert-butyloxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin + H2O | - |
Fasciola gigantica | tert-butyloxycarbonyl-L-Val-L-Leu-L-Lys + 7-amino-4-methylcoumarin | - |
? | |
tert-butyloxycarbonyl-L-Val-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Fasciola hepatica | tert-butyloxycarbonyl-L-Val-L-Pro-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
tert-butyloxycarbonyl-L-Val-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Fasciola gigantica | tert-butyloxycarbonyl-L-Val-L-Pro-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Fasciola hepatica | tosyl-Gly-L-Pro-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O | preferred substrate for isoform CatL1G | Fasciola gigantica | tosyl-Gly-L-Pro-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
type 1 collagen + H2O | efficient cleavage of type 1 collagen by isoform CatL5 is limited to the beta-and gamma-chains | Fasciola hepatica | ? | - |
? | |
type 1 collagen + H2O | isoform Catl1G completely digests collagen type 1 | Fasciola gigantica | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * about 38000, pro-enzyme, SDS-PAGE | Fasciola hepatica |
? | x * about 38000, pro-enzyme, SDS-PAGE | Fasciola gigantica |
Synonyms | Comment | Organism |
---|---|---|
CatL1G | juvenile isoform | Fasciola gigantica |
CatL5 | adult isoform | Fasciola hepatica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | - |
Fasciola hepatica |
6 | 7 | - |
Fasciola gigantica |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | after incubation for 2 h at 37°C in AMT buffers (100 mM sodium acetate, 100 mM MES, 200 mM Tris, 1 mM EDTA), the isoform CatL1G shows residual activities of about 30% at pH 4.0, about 75% at pH 5.0, about 85% at pH 6.0, about 45% at pH 7.0, and about 10% at pH 8.0-9.0. Isoform CatL1G retains 25% activity after 2 h at pH 7.5, but retains over 80% activity over 4 h at pH 5.5 and 50% over 24 h | Fasciola gigantica |
4 | 9 | isoform CatL5 undergoes significant autoprocessing if the pH of the yeast media is allowed to drop below pH 6.0. After incubation for 2 h at 37°C in AMT buffers (100 mM sodium acetate, 100 mM MES, 200 mM Tris, 1 mM EDTA), the isoform CatL5 shows residual activities of about 30% at pH 4.0-5.0, about 60% at pH 6.0, about 85% at pH 7.0, about 100% at pH 8.0, and about 60% at pH 9.0. Isoform CatL5 and mutant CatL5 L69W retain more than 90% activity after 4 h incubation at pH 7.5 and about 40% activity after incubation for 24 h | Fasciola hepatica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.01 | - |
tert-butyloxycarbonyl-L-Val-L-Pro-L-Arg-7-amido-4-methylcoumarin | wild type enzyme, at pH 5.5, in the presence of 10 mM cysteine-HCl, at 37°C | Fasciola gigantica | |
6.75 | - |
tert-butyloxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin | wild type enzyme, at pH 5.5, in the presence of 10 mM cysteine-HCl, at 37°C | Fasciola gigantica | |
10.1 | - |
tert-butyloxycarbonyl-L-Asp-L-Pro-L-Arg-7-amido-4-methylcoumarin | wild type enzyme, at pH 5.5, in the presence of 10 mM cysteine-HCl, at 37°C | Fasciola hepatica | |
11.3 | - |
tert-butyloxycarbonyl-L-Asp-L-Pro-L-Arg-7-amido-4-methylcoumarin | wild type enzyme, at pH 5.5, in the presence of 10 mM cysteine-HCl, at 37°C | Fasciola gigantica | |
16.6 | - |
tert-butyloxycarbonyl-L-Ala-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin | wild type enzyme, at pH 5.5, in the presence of 10 mM cysteine-HCl, at 37°C | Fasciola hepatica | |
18.1 | - |
benzoyl-L-Phe-L-Val-L-Arg-7-amido-4-methylcoumarin | wild type enzyme, at pH 5.5, in the presence of 10 mM cysteine-HCl, at 37°C | Fasciola gigantica | |
22.4 | - |
tert-butyloxycarbonyl-L-Val-L-Pro-L-Arg-7-amido-4-methylcoumarin | wild type enzyme, at pH 5.5, in the presence of 10 mM cysteine-HCl, at 37°C | Fasciola hepatica | |
24.4 | - |
tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin | wild type enzyme, at pH 5.5, in the presence of 10 mM cysteine-HCl, at 37°C | Fasciola hepatica | |
33.8 | - |
tert-butyloxycarbonyl-L-Ala-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin | wild type enzyme, at pH 5.5, in the presence of 10 mM cysteine-HCl, at 37°C | Fasciola gigantica | |
38.3 | - |
D-Val-Leu-Arg-7-amido-4-methylcoumarin | wild type enzyme, at pH 5.5, in the presence of 10 mM cysteine-HCl, at 37°C | Fasciola gigantica | |
38.5 | - |
tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin | wild type enzyme, at pH 5.5, in the presence of 10 mM cysteine-HCl, at 37°C | Fasciola gigantica | |
52.7 | - |
tert-butyloxycarbonyl-L-Val-L-Pro-L-Arg-7-amido-4-methylcoumarin | mutant enzyme L69W, at pH 5.5, in the presence of 10 mM cysteine-HCl, at 37°C | Fasciola hepatica | |
75.2 | - |
tert-butyloxycarbonyl-L-Asp-L-Pro-L-Arg-7-amido-4-methylcoumarin | mutant enzyme L69W, at pH 5.5, in the presence of 10 mM cysteine-HCl, at 37°C | Fasciola hepatica | |
84.3 | - |
tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin | mutant enzyme L69W, at pH 5.5, in the presence of 10 mM cysteine-HCl, at 37°C | Fasciola hepatica | |
119 | - |
tert-butyloxycarbonyl-L-Ala-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin | mutant enzyme L69W, at pH 5.5, in the presence of 10 mM cysteine-HCl, at 37°C | Fasciola hepatica | |
149 | - |
L-Pro-L-Phe-L-Arg-7-amido-4-methylcoumarin | wild type enzyme, at pH 5.5, in the presence of 10 mM cysteine-HCl, at 37°C | Fasciola hepatica | |
152 | - |
L-Phe-L-Arg-7-amido-4-methylcoumarin | mutant enzyme L69W, at pH 5.5, in the presence of 10 mM cysteine-HCl, at 37°C | Fasciola hepatica | |
182 | - |
benzoyl-L-Phe-L-Val-L-Arg-7-amido-4-methylcoumarin | mutant enzyme L69W, at pH 5.5, in the presence of 10 mM cysteine-HCl, at 37°C | Fasciola hepatica | |
211 | - |
L-Pro-L-Phe-L-Arg-7-amido-4-methylcoumarin | mutant enzyme L69W, at pH 5.5, in the presence of 10 mM cysteine-HCl, at 37°C | Fasciola hepatica | |
308 | - |
D-Val-Leu-Arg-7-amido-4-methylcoumarin | mutant enzyme L69W, at pH 5.5, in the presence of 10 mM cysteine-HCl, at 37°C | Fasciola hepatica | |
474 | - |
L-Phe-L-Arg-7-amido-4-methylcoumarin | wild type enzyme, at pH 5.5, in the presence of 10 mM cysteine-HCl, at 37°C | Fasciola hepatica | |
781 | - |
benzoyl-L-Phe-L-Val-L-Arg-7-amido-4-methylcoumarin | wild type enzyme, at pH 5.5, in the presence of 10 mM cysteine-HCl, at 37°C | Fasciola hepatica | |
1390 | - |
tert-butyloxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin | mutant enzyme L69W, at pH 5.5, in the presence of 10 mM cysteine-HCl, at 37°C | Fasciola hepatica | |
5420 | - |
D-Val-Leu-Arg-7-amido-4-methylcoumarin | wild type enzyme, at pH 5.5, in the presence of 10 mM cysteine-HCl, at 37°C | Fasciola hepatica | |
7220 | - |
tert-butyloxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin | wild type enzyme, at pH 5.5, in the presence of 10 mM cysteine-HCl, at 37°C | Fasciola hepatica |