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Literature summary for 3.4.22.15 extracted from

  • Huang, R.; Que, X.; Hirata, K.; Brinen, L.S.; Lee, J.H.; Hansell, E.; Engel, J.; Sajid, M.; Reed, S.
    The cathepsin L of Toxoplasma gondii (TgCPL) and its endogenous macromolecular inhibitor, toxostatin (2009), Mol. Biochem. Parasitol., 164, 86-94.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
-
 Toxoplasma gondii

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling of the mature domain using human cathepsin L as a template. Enzyme contains a non-canonical aspartic acid at the base of the predicted active site S2 pocket, which limits substrate access Toxoplasma gondii

Inhibitors

Inhibitors Comment Organism Structure
toxostatin endogenous inhibitor, acts both on cathepsin L and cathepsin B in the nanomolar range Toxoplasma gondii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
 Benzyloxycarbonyl-Lys-Arg pH 6.5 Toxoplasma gondii
0.0157
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin pH 6.5 Toxoplasma gondii
0.0176
-
 benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin pH 6.5 Toxoplasma gondii

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information enzyme has a putative transmembrane domain, which is cleaved from the mature enzyme. No membrane localization is detected Toxoplasma gondii
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 x * 32000, SDS-PAGE of partially matured protein, x * 24000, calculated for mautre protein Toxoplasma gondii
32000
-
 x * 32000, SDS-PAGE of partially matured protein, x * 24000, calculated for mautre protein Toxoplasma gondii

Organism

Organism UniProt Comment Textmining
Toxoplasma gondii
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification full-length zymogen consists of a deduced 221 amino acid mature protein, 125 amino acid proregion, and a 75 amino acid pre-region Toxoplasma gondii

Source Tissue

Source Tissue Comment Organism Textmining
tachyzoite primarily localized to its apical end, but does not co-localize with any apical organelles, including rhoptries, dense granules, or micronemes Toxoplasma gondii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Toxoplasma gondii ?
-
 ?
benzyloxycarbonyl-Lys-Arg + H2O
-
 Toxoplasma gondii ?
-
 ?
benzyloxycarbonyl-Lys-Gln-Lys-Leu-Arg-7-amido-4-methylcoumarin + H2O preferred substrate Toxoplasma gondii ?
-
 ?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Toxoplasma gondii ?
-
 ?
additional information leucine is preferred over phenylalanine in position P2 Toxoplasma gondii ?
-
 ?

Subunits

Subunits Comment Organism
? x * 32000, SDS-PAGE of partially matured protein, x * 24000, calculated for mautre protein Toxoplasma gondii

Synonyms

Synonyms Comment Organism
CPL
-
 Toxoplasma gondii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Toxoplasma gondii