Application | Comment | Organism |
---|---|---|
medicine | endothelial progenitor cells of patients with type 2 diabetes reveal profoundly decreased cathepsin L expression and activity | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
cell culture of endothelial progenitor cells, inhibitor glucose at higher concentrations dose-dependently decreases endothelial progenitor cells invasion, coincubation of glucose in higher concentrations and inhibitor N-benzyloxycarbonyl-Phe-Phe-fluoromethylketone does not decrease further endothelial progenitor cell invasion, high glucose concentrations decreases cathepsin L expression level but not cathepsin L mRNA level, reduced cathepsin L expression in patients with diabetes mellitus | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glucose | high concentrations | Homo sapiens | |
N-benzyloxycarbonyl-Phe-Phe-fluoromethylketone | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
mature form, SDS-PAGE, Western blot | Homo sapiens |
30000 | - |
mature form, SDS-PAGE, Western blot | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endothelial progenitor cell | - |
Homo sapiens | - |
endothelium | incubation of endothelial progenitor cells with high levels of glucose dose-dependently decreases cathepsin L activity and protein expression, while other proteases such as cathepsins D and O, and matrix metalloproteases MMP-2 and MMP-9, are not altered. Cathepsin L mRNA level is not affected | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
high glucose concentration dose-dependently reduces cathepsin L activity (up to approx. 65% of initial activity), dose range tested 5.5 mM to 30 mM, substrat N-benzyloxycarbonyl-Phe-Arg-4-methoxy-beta-naphthylamide, fluorogenic assay | Homo sapiens |
additional information | - |
high glucose concentrations dose-dependently reduce matrix degrading activity of endothelial progenitor cells, dose range tested 5.5 mM to 30 mM | Homo sapiens |
additional information | - |
reduced cathepsin L activity in patients with diabetes | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-benzyloxycarbonyl-Phe-Arg-2-(4-methoxynaphthylamide) + H2O | - |
Homo sapiens | N-benzyloxycarbonyl-Phe-Arg + 4-methoxy-2-naphthylamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cathepsin L | - |
Homo sapiens |