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Literature summary for 3.4.22.15 extracted from

  • Urbich, C.; Dernbach, E.; Roessig, L.; Zeiher, A.M.; Dimmeler, S.
    High glucose reduces cathepsin L activity and impairs invasion of circulating progenitor cells (2008), J. Mol. Cell. Cardiol., 45, 429-436.
    View publication on PubMed

Application

Application Comment Organism
medicine endothelial progenitor cells of patients with type 2 diabetes reveal profoundly decreased cathepsin L expression and activity Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
cell culture of endothelial progenitor cells, inhibitor glucose at higher concentrations dose-dependently decreases endothelial progenitor cells invasion, coincubation of glucose in higher concentrations and inhibitor N-benzyloxycarbonyl-Phe-Phe-fluoromethylketone does not decrease further endothelial progenitor cell invasion, high glucose concentrations decreases cathepsin L expression level but not cathepsin L mRNA level, reduced cathepsin L expression in patients with diabetes mellitus Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
glucose high concentrations Homo sapiens
N-benzyloxycarbonyl-Phe-Phe-fluoromethylketone
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
mature form, SDS-PAGE, Western blot Homo sapiens
30000
-
mature form, SDS-PAGE, Western blot Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
endothelial progenitor cell
-
Homo sapiens
-
endothelium incubation of endothelial progenitor cells with high levels of glucose dose-dependently decreases cathepsin L activity and protein expression, while other proteases such as cathepsins D and O, and matrix metalloproteases MMP-2 and MMP-9, are not altered. Cathepsin L mRNA level is not affected Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
high glucose concentration dose-dependently reduces cathepsin L activity (up to approx. 65% of initial activity), dose range tested 5.5 mM to 30 mM, substrat N-benzyloxycarbonyl-Phe-Arg-4-methoxy-beta-naphthylamide, fluorogenic assay Homo sapiens
additional information
-
high glucose concentrations dose-dependently reduce matrix degrading activity of endothelial progenitor cells, dose range tested 5.5 mM to 30 mM Homo sapiens
additional information
-
reduced cathepsin L activity in patients with diabetes Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-benzyloxycarbonyl-Phe-Arg-2-(4-methoxynaphthylamide) + H2O
-
Homo sapiens N-benzyloxycarbonyl-Phe-Arg + 4-methoxy-2-naphthylamine
-
?

Synonyms

Synonyms Comment Organism
cathepsin L
-
Homo sapiens