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Literature summary for 3.4.22.15 extracted from
- Purification and characterization of cathepsin L from the muscle of silver carp (Hypophthalmichthys molitrix) (2006), J. Agric. Food Chem., 54, 9584-9591.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | 172% activity in the presence of 2 mM dithiothreitol and 1 mM EDTA, dithiothreitol alone does not inhibit activity | Hypophthalmichthys molitrix |  |
| L-cysteine | 114% activity at 5 mM | Hypophthalmichthys molitrix | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 50% activity at 2 mM | Hypophthalmichthys molitrix | |
| Ca2+ | 51% residual activity at 1 mM | Hypophthalmichthys molitrix | |
| Co2+ | complete inhibition at 1 mM | Hypophthalmichthys molitrix | |
| Cu2+ | complete inhibition at 1 mM | Hypophthalmichthys molitrix | |
| E-64 | complete inhibition at 0.1 mM | Hypophthalmichthys molitrix | |
| EDTA | 98% residual activity at 2 mM | Hypophthalmichthys molitrix | |
| Fe2+ | 5% residual activity at 1 mM | Hypophthalmichthys molitrix | |
| Fe3+ | complete inhibition at 1 mM | Hypophthalmichthys molitrix | |
| Mg2+ | 66% residual activity at 1 mM | Hypophthalmichthys molitrix | |
| Mn2+ | 65% residual activity at 1 mM | Hypophthalmichthys molitrix | |
| additional information | insensitive to K+, phenylmethylsulfonylfluoride and pepstatin A | Hypophthalmichthys molitrix | |
| Ni2+ | complete inhibition at 1 mM | Hypophthalmichthys molitrix | |
| Zn2+ | 10% residual activity at 1 mM | Hypophthalmichthys molitrix | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00825 | - |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | - |
Hypophthalmichthys molitrix | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
SDS-PAGE | Hypophthalmichthys molitrix |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hypophthalmichthys molitrix | - |
silver carp | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, DEAE-Sephacel gel filtration, Sephacryl S-100 gel filtration, SP Sepharose fast flow chromatography, and Con A Sepharose chromatography | Hypophthalmichthys molitrix |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| muscle | - |
Hypophthalmichthys molitrix | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.12 | - |
crude extract | Hypophthalmichthys molitrix |
| 6 | - |
after 48.4fold purification | Hypophthalmichthys molitrix |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin + H2O | 7% activity compared to benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | Hypophthalmichthys molitrix | benzyloxycarbonyl-Arg + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O | 9% activity compared to benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | Hypophthalmichthys molitrix | benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | 100% activity | Hypophthalmichthys molitrix | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
| surimi protein + H2O | - |
Hypophthalmichthys molitrix | ? | - |
? | |
| tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O | 1% activity compared to benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | Hypophthalmichthys molitrix | tert-butyloxycarbonyl-Val-Leu-Lys + 7-amino-4-methylcoumarin | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cathepsin L1 | - |
Hypophthalmichthys molitrix |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Hypophthalmichthys molitrix |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 65 | the enzyme exhibits a higher hydrolytic activity on surimi protein at 65°C than at 40°C | Hypophthalmichthys molitrix |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | 3.7 | benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | - |
Hypophthalmichthys molitrix | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Hypophthalmichthys molitrix |
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