Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 325% activity at 2 mM dithiothreitol | Gadus chalcogrammus | |
proteinase inhibitor for serine protease | 113% activity at 0.1 g/l | Gadus chalcogrammus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetate | complete inhibition at 1 mM | Gadus chalcogrammus | |
L-trans-epoxy-succinyl-leucylamido-(4-guanidino)-butane | complete inhibition at 0.1 mM | Gadus chalcogrammus | |
leupeptin | complete inhibition at 0.1 mM | Gadus chalcogrammus | |
additional information | not inhibited by EDTA | Gadus chalcogrammus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gadus chalcogrammus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Sepharose 6B column chromatography | Gadus chalcogrammus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin + H2O | - |
Gadus chalcogrammus | benzyloxycarbonyl-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O | - |
Gadus chalcogrammus | benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Gadus chalcogrammus | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
myosin heavy chain + H2O | - |
Gadus chalcogrammus | ? | - |
? | |
tert-butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O | - |
Gadus chalcogrammus | tert-butyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
after incubation at 25°C for 120 min 73-86% of the original activity remain | Gadus chalcogrammus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | at pH 4.0 and 5.0 activity decreases slowly and remains at 38.86% and 48.54%, respectively, after incubation at 45°C for 120 min, at pH 7.0, activity of cathepsin L decreases sharply to about 10% residual activity after incubation at 45°C for 10 min | Gadus chalcogrammus |