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Literature summary for 3.4.22.15 extracted from

  • Aranishi, F.; Ogata, H.; Hara, K.; Osatomi, K.; Ishihara, T.M.
    Purification and characterization of cathepsin L from hepatopancreas of carp Cyprinus carpio (1997), Comp. Biochem. Physiol. B, 118, 531-537.
    View publication on PubMed
Search for more literature for 3.4.22.15

Activating Compound

Activating Compound Comment Organism Structure
Cys activates Rattus norvegicus
Cys activates Cyprinus carpio

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Cyprinus carpio
5,5'-dithiobis(2-nitrobenzoic acid)
-
 Cyprinus carpio
5,5'-dithiobis(2-nitrobenzoic acid)
-
 Rattus norvegicus
AgNO3
-
 Cyprinus carpio
antipain
-
 Cyprinus carpio
CdCl2
-
 Cyprinus carpio
chymostatin
-
 Cyprinus carpio
CoCl2 partial Cyprinus carpio
CuCl2
-
 Cyprinus carpio
FeCl3
-
 Cyprinus carpio
Hg2+ HgCl2 Cyprinus carpio
Hg2+
-
 Rattus norvegicus
leupeptin
-
 Cyprinus carpio
leupeptin
-
 Rattus norvegicus
PCMB
-
 Cyprinus carpio
PCMB
-
 Rattus norvegicus
SnCl2 < partial Cyprinus carpio
trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane
-
 Cyprinus carpio
trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane i.e. E-64, in vivo, the inhibitor is transported to the liver cytosol in the free form in the blood and permeated into the lysosomes, where it binds to, and inactivates the enzyme Rattus norvegicus
Zn(CH3COO)2
-
 Cyprinus carpio

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03416
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
-
 Cyprinus carpio
0.04371
-
 Pro-Phe-Arg-4-methylcoumarin 7-amide
-
 Cyprinus carpio
0.1417
-
 tert-butyloxycarbonyl-Val-Leu-Lys-4-methylcoumarin 7-amide
-
 Cyprinus carpio

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Rattus norvegicus 5764
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 x * 24000 + x * 30000, SDS-PAGE under reducing conditions Cyprinus carpio
30000
-
 gel filtration Cyprinus carpio

Organism

Organism UniProt Comment Textmining
Cyprinus carpio
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cyprinus carpio

Source Tissue

Source Tissue Comment Organism Textmining
hepatopancreas
-
 Cyprinus carpio
-
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Cyprinus carpio

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
-
 Rattus norvegicus ?
-
 ?
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
-
 Cyprinus carpio ?
-
 ?
Pro-Phe-Arg-4-methylcoumarin 7-amide + H2O
-
 Cyprinus carpio ?
-
 ?
tert-butyloxycarbonyl-Val-Leu-Lys-4-methylcoumarin 7-amide + H2O
-
 Cyprinus carpio ?
-
 ?

Subunits

Subunits Comment Organism
?
-
 Rattus norvegicus
? x * 24000 + x * 30000, SDS-PAGE under reducing conditions Cyprinus carpio

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 hydrolysis of benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide Cyprinus carpio

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30
-
 less than half maximal activity below 30°C and above 60°C Cyprinus carpio

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable below Cyprinus carpio

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.52
-
 Pro-Phe-Arg-4-methylcoumarin 7-amide
-
 Cyprinus carpio
1.67
-
 tert-butyloxycarbonyl-Val-Leu-Lys-4-methylcoumarin 7-amide
-
 Cyprinus carpio
9.5
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
-
 Cyprinus carpio

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6 hydrolysis of benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide Cyprinus carpio

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 6.5 stable Cyprinus carpio