Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.22.1 extracted from

  • Han, Y.P.; Li, Z.Y.; Li, B.C.; Sun, X.; Zhu, C.C.; Ling, X.T.; Zheng, H.Q.; Wu, Z.D.; Lv, Z.Y.
    Molecular cloning and characterization of a cathepsin B from Angiostrongylus cantonensis (2011), Parasitol. Res., 109, 369-378.
    View publication on PubMed
Search for more literature for 3.4.22.1

Activating Compound

Activating Compound Comment Organism Structure
L-cysteine cathepsin B is activated at 22°C for 5 min in 100 mM sodium acetate, 1 mM DTT, 2 mM EDTA, 4 mM cysteine, pH 5.5 Angiostrongylus cantonensis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Angiostrongylus cantonensis

Inhibitors

Inhibitors Comment Organism Structure
cystatin the enzyme retains 10% and 5% of its initial activity, after 30 min of incubation at 37°C, in the presence of 75 and 100 nM recombinant cystatin, respectively Angiostrongylus cantonensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 x * 40000, SDS-PAGE Angiostrongylus cantonensis
40850
-
 x * 40850, calculated from amino acid sequence Angiostrongylus cantonensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ovalbumin + H2O Angiostrongylus cantonensis
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Angiostrongylus cantonensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon affinity column chromatography Angiostrongylus cantonensis

Source Tissue

Source Tissue Comment Organism Textmining
adult
-
 Angiostrongylus cantonensis
-
larva the enzyme is expressed significantly higher in the fourth-stage larvae and the fifth-stage larvae than that in the third-stage larvae and adult worms Angiostrongylus cantonensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin + H2O
-
 Angiostrongylus cantonensis N-benzyloxycarbonyl-L-Arg-L-Arg + 7-amino-4-methylcoumarin
-
 ?
ovalbumin + H2O
-
 Angiostrongylus cantonensis ?
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Angiostrongylus cantonensis
? x * 40850, calculated from amino acid sequence Angiostrongylus cantonensis

Synonyms

Synonyms Comment Organism
CPB
-
 Angiostrongylus cantonensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Angiostrongylus cantonensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
17 57 the activity is relatively stable at 17°C and 27°C, retaining about 95% and 98% activity, respectively. The relative activities at 47°C and 57°C are 31% and 13% of that at 37°C, respectively Angiostrongylus cantonensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
99
-
 the activity drops dramatically within the first 30 min and 70% activity is lost after 30 min of incubation at 99°C Angiostrongylus cantonensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Angiostrongylus cantonensis

pH Range

pH Minimum pH Maximum Comment Organism
4 6 there is 60% activity at pH 4.0. The activity decreases significantly below pH 4.0 or above pH 5.0, and 5% activity is detected at pH 6.0 Angiostrongylus cantonensis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.06
-
 at pH 5.0 and 37°C Angiostrongylus cantonensis cystatin