Literature summary for 3.4.21.B48 extracted from

Wlodawer, A.; Li, M.; Gustchina, A.; Tsuruoka, N.; Ashida, M.; Minakata, H.; Oyama, H.; Oda, K.; Nishino, T.; Nakayama, T.
Crystallographic and biochemical investigations of kumamolisin-AS a serine-carboxyl peptidase with collagenase activity (2004), J. Biol. Chem., 279, 21500-21510.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure of the uninhibited enzyme and its complex with the covalently bound inhibitor, N-acetyl-Ile-Pro-phenylalaninal, vapor diffusion method	Alicyclobacillus sendaiensis

Protein Variants

Protein Variants	Comment	Organism
E78H	0.01% activity compared to wild-type enzyme, the canonical catalytic triad is disrupted	Alicyclobacillus sendaiensis

Inhibitors

Inhibitors	Comment	Organism	Structure
N-acetyl-Ile-Pro-Phe	-	Alicyclobacillus sendaiensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0007	-	[2-(N-methylamino)benzoyl]-MGPHFFP-Nepsilon-(2,4-dinitrophenyl)-L-Lys-D-Arg-D-Arg-amide	mutant enzyme E78H	Alicyclobacillus sendaiensis

Organism

Organism	UniProt	Comment	Textmining
Alicyclobacillus sendaiensis	-	NTAP-2	-
Alicyclobacillus sendaiensis NTAP-2	-	NTAP-2	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
[2-(N-methylamino)benzoyl]-MGPHFFP-Nepsilon-(2,4-dinitrophenyl)-L-Lys-D-Arg-D-Arg-amide + H2O	-	Alicyclobacillus sendaiensis	[2-(N-methylamino)benzoyl]-MGPH + FFP-Nepsilon-(2,4-dinitrophenyl)-L-Lys-D-Arg-D-Arg-amide	-	?
[2-(N-methylamino)benzoyl]-MGPHFFP-Nepsilon-(2,4-dinitrophenyl)-L-Lys-D-Arg-D-Arg-amide + H2O	-	Alicyclobacillus sendaiensis NTAP-2	[2-(N-methylamino)benzoyl]-MGPH + FFP-Nepsilon-(2,4-dinitrophenyl)-L-Lys-D-Arg-D-Arg-amide	-	?

Synonyms

Synonyms	Comment	Organism
kumamolisin-AS	-	Alicyclobacillus sendaiensis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.033	-	[2-(N-methylamino)benzoyl]-MGPHFFP-Nepsilon-(2,4-dinitrophenyl)-L-Lys-D-Arg-D-Arg-amide	mutant enzyme E78H	Alicyclobacillus sendaiensis

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Release 2023.1 (January 2023)