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Literature summary for 3.4.21.89 extracted from

  • Bockstael, K.; Geukens, N.; Van Mellaert, L.; Herdewijn, P.; Anne, J.; Van Aerschot, A.
    Evaluation of the type I signal peptidase as antibacterial target for biofilm-associated infections of Staphylococcus epidermidis (2009), Microbiology, 155, 3719-3729.
    View publication on PubMed

Application

Application Comment Organism
drug development in the search for antibacterial therapies, the type I signal peptidase serves as a potential target for development of antibacterials with another mode of action. SPase I is also is a feasible target for biofilm-associated infections Staphylococcus epidermidis

Cloned(Commentary)

Cloned (Comment) Organism
genes sip2 and sip3, expression of His-tagged isozymes Sip2 and Sip3 in Escherichia coli strain BL21(DE3) Staphylococcus epidermidis

Inhibitors

Inhibitors Comment Organism Structure
arylomycin A2 a lipohexapeptide SPase I inhibitor Staphylococcus epidermidis
rifampicin
-
Staphylococcus epidermidis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Staphylococcus epidermidis the enzyme cleaves off the signal peptide from secreted proteins, making it essential for protein secretion, and hence for bacterial cell viability ?
-
?

Organism

Organism UniProt Comment Textmining
Staphylococcus epidermidis
-
strain ATCC 12228, isozymes Sip2 and Sip3, genes sip2 and sip3
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged Sip2 and Sip3 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Staphylococcus epidermidis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Dabcyl-AGHDAHASET(EDANS) + H2O a substrate constructed based on the C-terminal region of the Staphylococcus epidermidis pre-SceD protein and containing the native SPase I cleavage site Staphylococcus epidermidis ?
-
?
additional information the enzyme cleaves off the signal peptide from secreted proteins, making it essential for protein secretion, and hence for bacterial cell viability Staphylococcus epidermidis ?
-
?
additional information measurement of enzyme activity using fluorescence resonance energy transfer-based assay Staphylococcus epidermidis ?
-
?

Synonyms

Synonyms Comment Organism
Sip2
-
Staphylococcus epidermidis
Sip3
-
Staphylococcus epidermidis
Spase I
-
Staphylococcus epidermidis
type I signal peptidase
-
Staphylococcus epidermidis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Staphylococcus epidermidis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Staphylococcus epidermidis

General Information

General Information Comment Organism
physiological function the enzyme cleaves off the signal peptide from secreted proteins, making it essential for protein secretion, and hence for bacterial cell viability Staphylococcus epidermidis