Application | Comment | Organism |
---|---|---|
drug development | in the search for antibacterial therapies, the type I signal peptidase serves as a potential target for development of antibacterials with another mode of action. SPase I is also is a feasible target for biofilm-associated infections | Staphylococcus epidermidis |
Cloned (Comment) | Organism |
---|---|
genes sip2 and sip3, expression of His-tagged isozymes Sip2 and Sip3 in Escherichia coli strain BL21(DE3) | Staphylococcus epidermidis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arylomycin A2 | a lipohexapeptide SPase I inhibitor | Staphylococcus epidermidis | |
rifampicin | - |
Staphylococcus epidermidis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Staphylococcus epidermidis | the enzyme cleaves off the signal peptide from secreted proteins, making it essential for protein secretion, and hence for bacterial cell viability | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus epidermidis | - |
strain ATCC 12228, isozymes Sip2 and Sip3, genes sip2 and sip3 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged Sip2 and Sip3 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Staphylococcus epidermidis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Dabcyl-AGHDAHASET(EDANS) + H2O | a substrate constructed based on the C-terminal region of the Staphylococcus epidermidis pre-SceD protein and containing the native SPase I cleavage site | Staphylococcus epidermidis | ? | - |
? | |
additional information | the enzyme cleaves off the signal peptide from secreted proteins, making it essential for protein secretion, and hence for bacterial cell viability | Staphylococcus epidermidis | ? | - |
? | |
additional information | measurement of enzyme activity using fluorescence resonance energy transfer-based assay | Staphylococcus epidermidis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Sip2 | - |
Staphylococcus epidermidis |
Sip3 | - |
Staphylococcus epidermidis |
Spase I | - |
Staphylococcus epidermidis |
type I signal peptidase | - |
Staphylococcus epidermidis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Staphylococcus epidermidis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Staphylococcus epidermidis |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme cleaves off the signal peptide from secreted proteins, making it essential for protein secretion, and hence for bacterial cell viability | Staphylococcus epidermidis |