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Literature summary for 3.4.21.89 extracted from

  • Engel, L.S.; Hill, J.M.; Caballero, A.R.; Green, L.C.; O'Callaghan, R.J.
    Protease IV, a unique extracellular protease and virulence factor from Pseudomonas aeruginosa (1998), J. Biol. Chem., 273, 16792-16797.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Aprotinin
-
Pseudomonas aeruginosa
bestatin
-
Pseudomonas aeruginosa
EDTA
-
Pseudomonas aeruginosa
EGTA
-
Pseudomonas aeruginosa
Elastinal
-
Pseudomonas aeruginosa

Application

Application Comment Organism
medicine developing of medication designed to arrest tissue damage during Pseudomonas infection, opportunistic pathogen causes morbidity and mortality in patients with burns, cystic fibrosis, pneumonia, urinary tract infections, skin infections, cancer, acquired immunodeficiency syndrome, and ocular disease Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment) Organism
-
Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
beta-mercaptoethanol
-
Pseudomonas aeruginosa
diisopropyl fluorophosphate partial inhibition Pseudomonas aeruginosa
dithiothreitol
-
Pseudomonas aeruginosa
additional information not inhibited by thiol-, carboxyl-, or metalloproteinase inhibitors Pseudomonas aeruginosa
N-[(1S)-1-(chloroacetyl)-3-methylbutyl]-4-methylbenzenesulfonamide total loss of enzyme activity Pseudomonas aeruginosa
phenylmethyl sulfonyl fluoride partial inhibition Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.319
-
tosyl-Gly-Pro-Lys-p-nitroanilide pH 10.0, 25°C Pseudomonas aeruginosa
0.727
-
Val-Leu-Lys-p-nitroanilide pH 10.0, 25°C Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Pseudomonas aeruginosa
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
-
Pseudomonas aeruginosa
26380
-
mass spectrometry Pseudomonas aeruginosa
30000
-
gel filtration, SDS-PAGE Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
signal peptides from preproteins + H2O Pseudomonas aeruginosa
-
mature proteins
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-
Pseudomonas aeruginosa PA103-29
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
specific activity 471 units/mg, 1 unit is DELTA A/min x total assay volume/sample volume x E 410 nm x light path (cm) where the extinction coefficient E of the product p-nitroanilide at 410 nm is 9.75 and the light path is 0.53 cm Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
complement component C1q + H2O partially degraded Pseudomonas aeruginosa ?
-
?
Fibrinogen + H2O
-
Pseudomonas aeruginosa ?
-
?
Fibrinogen + H2O
-
Pseudomonas aeruginosa PA103-29 ?
-
?
IgG + H2O partially degraded Pseudomonas aeruginosa ?
-
?
Immunoglobulin + H2O
-
Pseudomonas aeruginosa ?
-
?
Immunoglobulin + H2O
-
Pseudomonas aeruginosa PA103-29 ?
-
?
additional information leucine-4-nitroanilide, carbobenzoxy-L-phenylalanyl-L-leucyl-L-alpha-glutamyl-4-nitroanilide, Z-Val-Gly-Arg-4-nitroanilide, benzoyl-beta-alanyl-glycyl-arginine-4-nitroanilide, tosyl-glycyl-prolyl-arginine-4-nitroanilide, and L-Lys-4-nitroanilide are no substrates Pseudomonas aeruginosa ?
-
?
additional information leucine-4-nitroanilide, carbobenzoxy-L-phenylalanyl-L-leucyl-L-alpha-glutamyl-4-nitroanilide, Z-Val-Gly-Arg-4-nitroanilide, benzoyl-beta-alanyl-glycyl-arginine-4-nitroanilide, tosyl-glycyl-prolyl-arginine-4-nitroanilide, and L-Lys-4-nitroanilide are no substrates Pseudomonas aeruginosa PA103-29 ?
-
?
plasmin + H2O partially degraded Pseudomonas aeruginosa ?
-
?
plasmin + H2O partially degraded Pseudomonas aeruginosa PA103-29 ?
-
?
plasminogen + H2O
-
Pseudomonas aeruginosa ?
-
?
signal peptides from preproteins + H2O
-
Pseudomonas aeruginosa mature proteins
-
?
tosyl-Gly-Pro-Lys-p-nitroanilide + H2O chromozym PL Pseudomonas aeruginosa tosyl-Gly-Pro-Lys + p-nitroaniline
-
?
Val-Leu-Lys-p-nitroanilide + H2O chromozym PL Pseudomonas aeruginosa Val-Leu-Lys + p-nitroaniline
-
?
Val-Leu-Lys-p-nitroanilide + H2O chromozym PL Pseudomonas aeruginosa PA103-29 Val-Leu-Lys + p-nitroaniline
-
?

Subunits

Subunits Comment Organism
monomer 1 * 30000, SDS-PAGE Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
protease IV
-
Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Pseudomonas aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 80 90% of the enzyme activity is abolished by incubation at 60°C for 40 min or 80°C for 1 min Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
Pseudomonas aeruginosa

pH Range

pH Minimum pH Maximum Comment Organism
4 11 activity increases from pH 4.0-9.0, 85% of maximal activity at pH 11.0, no activity above pH 12.0 Pseudomonas aeruginosa

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas aeruginosa isoelectric focusing
-
8.7