KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
alkaline phosphatase signal peptide fused to full-length mammalian cytochrome b5 | pH 7.5, 37°C | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | integral component of the cytoplasmic membrane | Escherichia coli | 5737 | - |
plasma membrane | integral membrane protein | Escherichia coli | 5886 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
signal peptides from preproteins + H2O | Escherichia coli | in vivo, type I signal peptidase is the principal peptidase responsible for signal peptide cleavage as pre-proteins of a number of exported proteins, proteins designed for transport across the cytoplasmic membrane are generally synthesised as precursors with cleavable signal peptides in the cytoplasm, the signal peptides targets the pre-proteins to the respective translocase, during or shortly after translocation across the cytoplasmic membrane, the signal peptide is enzymatically removed | mature proteins | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alkaline phosphatase signal peptide fused to full-length mammalian cytochrome b5 | amphipatic, chimeric cytochrome b5 precursor | Escherichia coli | cytochrome b5 | - |
? | |
pro-ompA-nuclease + H2O | - |
Escherichia coli | ompA-nuclease + ? | - |
? | |
signal peptides from preproteins + H2O | - |
Escherichia coli | mature proteins | - |
? | |
signal peptides from preproteins + H2O | in vivo, type I signal peptidase is the principal peptidase responsible for signal peptide cleavage as pre-proteins of a number of exported proteins, proteins designed for transport across the cytoplasmic membrane are generally synthesised as precursors with cleavable signal peptides in the cytoplasm, the signal peptides targets the pre-proteins to the respective translocase, during or shortly after translocation across the cytoplasmic membrane, the signal peptide is enzymatically removed | Escherichia coli | mature proteins | - |
? |
Synonyms | Comment | Organism |
---|---|---|
signal peptidase I | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
alkaline phosphatase signal peptide fused to full-length mammalian cytochrome b5 | pH 7.5, 37°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | maximal processing rates at pH 8.5 and above, 90% activity at neutral pH | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 9 | 20% of maximal activity at pH 4.5, half-maximal activity at pH 6.5, 90% of maximal activity at pH 7.0 | Escherichia coli |