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Literature summary for 3.4.21.89 extracted from

  • Stein, R.L.; Barbosa, M.D.F.S.; Bruckner, R.
    Kinetic and mechanistic studies of signal peptidase I from Escherichia coli (2000), Biochemistry, 39, 7973-7983.
    View publication on PubMed
Search for more literature for 3.4.21.89

Cloned(Commentary)

Cloned (Comment) Organism
lepB gene PCR amplified Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
(NO2)YFSASALA product inhibition Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0006
-
 Y-NO2-FSASALAKIK-2-aminobenzoyl-NH2 pH 8.1, 37°C Escherichia coli
0.0328
-
 YFSASALA-4-methylcoumarin 7-amide pH 8.1, 37°C Escherichia coli
0.0339
-
 YFSASALA-4-methylcoumarin 7-amide pH 8.1, 37°C, nD2O 0.21 Escherichia coli
0.034
-
 YFSASALA-4-methylcoumarin 7-amide pH 8.1, 37°C, nD2O 0.43 Escherichia coli
0.0347
-
 YFSASALA-4-methylcoumarin 7-amide pH 8.1, 37°C, nD2O 0.64 Escherichia coli
0.0354
-
 YFSASALA-4-methylcoumarin 7-amide pH 8.1, 37°C, nD2O 0.85 Escherichia coli
0.11
-
 Y-NO2-FSASALAKIK-2-aminobenzoyl-NH2 pH 8.1, 37°C Escherichia coli
0.8
-
 FSASALAKI pH 8.1, 37°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
signal peptides from preproteins + H2O Escherichia coli key role in the protein secretary pathway mature proteins
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(NO2)YFSASALA-KI-(2-aminobenzoyl)K-NH2 + H2O
-
 Escherichia coli (NO2)YFSASALA + KI-(2-aminobenzoyl)K-NH2
-
 ?
FSASALAKI + H2O most rapidly cleaved peptide Escherichia coli FSASALA + Lys-Ile
-
 ?
signal peptides from preproteins + H2O
-
 Escherichia coli mature proteins
-
 ?
signal peptides from preproteins + H2O key role in the protein secretary pathway Escherichia coli mature proteins
-
 ?
Y-NO2-FSASALAKIK-2-aminobenzoyl-NH2 + H2O
-
 Escherichia coli Y-NO2-FSASALA + KIK-2-aminobenzoyl-NH2
-
 ?
YFSASALA-4-methylcoumarin-7-amide + H2O
-
 Escherichia coli YFSASALA + 7-amino-4-methylcoumarin
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00028
-
 YFSASALA-4-methylcoumarin 7-amide pH 8.1, 37°C, nD2O 0.85 Escherichia coli
0.00034
-
 YFSASALA-4-methylcoumarin 7-amide pH 8.1, 37°C, nD2O 0.64 Escherichia coli
0.000407
-
 YFSASALA-4-methylcoumarin 7-amide pH 8.1, 37°C, nD2O 0.43 Escherichia coli
0.000467
-
 YFSASALA-4-methylcoumarin 7-amide pH 8.1, 37°C, nD2O 0.21 Escherichia coli
0.000528
-
 YFSASALA-4-methylcoumarin 7-amide pH 8.1, 37°C Escherichia coli
0.001 0.01 Y-NO2-FSASALAKIK-2-aminobenzoyl-NH2 pH 8.1, 37°C Escherichia coli
0.03
-
 FSASALA-KI pH 8.1, 37°C Escherichia coli
1.5
-
 Y-NO2-FSASALAKIK-2-aminobenzoyl-NH2 pH 8.1, 37°C Escherichia coli