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Literature summary for 3.4.21.89 extracted from

  • Dalbey, R.E.; von Heijne, G.
    Signal peptidases in prokaryotes and eukaryotes--a new protease family [published erratum appears in Trends Biochem Sci 1993 Jan;18(1):25] (1992), Trends Biochem. Sci., 17, 474-478.
    View publication on PubMed
Search for more literature for 3.4.21.89

Cloned(Commentary)

Cloned (Comment) Organism
19 kDa subunit encoded by SEC11 gene Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by classical protease inhibitors such as phenylmethyl sulfonyl fluoride, tosyl-amido-2-phenylethylchloromethylketone, EDTA, o-phenanthroline, N-ethylmaleimide, dinitrophenol, carboxyphenanthroline, or 2,6-pyridinecarboxylic acid Escherichia coli
Signal peptides that include a Pro residue at position +1
-
 Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm integral component of the cytoplasmic membrane Escherichia coli 5737
-
membrane integral membrane protein Gallus gallus 16020
-
membrane integral membrane protein Escherichia coli 16020
-
membrane integral membrane protein Saccharomyces cerevisiae 16020
-
mitochondrion inner membrane Saccharomyces cerevisiae 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 active monomer, SDS-PAGE Pseudomonas fluorescens
36000
-
 active monomer, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
37000
-
 active monomer, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
signal peptides from preproteins + H2O Salmonella enterica subsp. enterica serovar Typhimurium
-
 mature proteins
-
 ?
signal peptides from preproteins + H2O Escherichia coli
-
 mature proteins
-
 ?
signal peptides from preproteins + H2O Pseudomonas fluorescens
-
 mature proteins
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Gallus gallus
-
-
-
Pseudomonas fluorescens
-
-
-
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae
-
 yeast
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Reaction

Reaction Comment Organism Reaction ID
Cleavage of hydrophobic, N-terminal signal or leader sequences mechanism Gallus gallus
a
Cleavage of hydrophobic, N-terminal signal or leader sequences mechanism Escherichia coli
a
Cleavage of hydrophobic, N-terminal signal or leader sequences mechanism Saccharomyces cerevisiae
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information specificity overview Gallus gallus ?
-
 ?
additional information specificity overview Escherichia coli ?
-
 ?
additional information specificity overview Saccharomyces cerevisiae ?
-
 ?
additional information cleavage of E. coli enzyme requires a small residue at-1 and a small or aliphatic residue at-3, presence of a helix breaker allows the pre-protein to bind with higher affinity, signal peptides that include a Pro residue at position-1 are not cleaved in E. coli Escherichia coli ?
-
 ?
signal peptides from preproteins + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium mature proteins
-
 ?
signal peptides from preproteins + H2O
-
 Escherichia coli mature proteins
-
 ?
signal peptides from preproteins + H2O
-
 Pseudomonas fluorescens mature proteins
-
 ?