Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | above 1 mM | Escherichia coli | |
additional information | but site-directed mutagenesis implicates a Ser/Lys catalytic dyad in activity; unaffected by inhibitors of most serine peptidases | Escherichia coli | |
sodium chloride | above 160 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile | - |
Escherichia coli | |
1 | - |
Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile-NH2 | - |
Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | integral membrane protein | Escherichia coli | 16020 | - |
membrane | it is anchored to the membrane by two transmembrane segments and is oriented with a short domain facing the cytoplasm and a large carboxyl-terminal domain exposed to the periplasmic space | Escherichia coli | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
1 * 36000 | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | the physiological role is to release exported proteins from the membrane by removing the leader sequence | ? | - |
? | |
additional information | Escherichia coli MC1061/pRD8 | the physiological role is to release exported proteins from the membrane by removing the leader sequence | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli MC1061/pRD8 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
M13 phage procoat protein + H2O | hydrolysis of a single-Ala-+-Ala- bond, the term-+- depicts the point of cleavage | Escherichia coli | Free signal peptide + coat protein | - |
? | |
M13 phage procoat protein + H2O | hydrolysis of a single-Ala-+-Ala- bond, the term-+- depicts the point of cleavage | Escherichia coli MC1061/pRD8 | Free signal peptide + coat protein | - |
? | |
additional information | specificity: leader peptidase 1 cleaves the majority of the preproteins destined to the cell surface | Escherichia coli | ? | - |
? | |
additional information | the physiological role is to release exported proteins from the membrane by removing the leader sequence | Escherichia coli | ? | - |
? | |
additional information | specificity: leader peptidase 1 cleaves the majority of the preproteins destined to the cell surface | Escherichia coli MC1061/pRD8 | ? | - |
? | |
additional information | the physiological role is to release exported proteins from the membrane by removing the leader sequence | Escherichia coli MC1061/pRD8 | ? | - |
? | |
Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile + H2O | - |
Escherichia coli | Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile + ? | - |
? | |
Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile-NH2 + H2O | - |
Escherichia coli | Phe-Ser-Ala-Ser-Ala-Leu-Ala + Lys-Ile-NH2 | - |
? | |
Pre-beta-lactamase + H2O | - |
Escherichia coli | beta-Lactamase + ? | - |
? | |
Pre-beta-lactamase + H2O | - |
Escherichia coli MC1061/pRD8 | beta-Lactamase + ? | - |
? | |
Pre-lambda phage receptor + H2O | - |
Escherichia coli | Lambda Phage receptor + ? | - |
? | |
Precursor of the leucine-binding protein + H2O | - |
Escherichia coli | Leucine-binding protein + ? | - |
? | |
Precursor of the leucine-binding protein + H2O | - |
Escherichia coli MC1061/pRD8 | Leucine-binding protein + ? | - |
? | |
Premaltose-binding protein + H2O | - |
Escherichia coli | Maltose-binding protein + ? | - |
? | |
Pro-OmpA + H2O | - |
Escherichia coli | OmpA + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 36000 | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0212 | - |
Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile | - |
Escherichia coli | |
0.033 | - |
Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile-NH2 | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 6.9 | Escherichia coli |