KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0022 | - |
human titin | unfolded, bearing a sul20C peptide variant with an N-terminal PABA fluorophoreand a nitrotyrosine quencher at the penultimate C-terminal position followed by alanine at the C-terminus of the I27 domain, pH 8.0, 37°C | Escherichia coli | |
0.024 | - |
human titin | unfolded, bearing a 20-residue beta-galactosidase sequence at the C-terminus of the I27 domain, pH 8.0, 37°C | Escherichia coli | |
0.04 | - |
human titin | native, bearing C-terminal 20 residues of SulA sequence at the C-terminus of the I27 domain, pH 8.0, 37°C | Escherichia coli | |
0.05 | - |
human titin | native, bearing a 20-residue beta-galactosidase sequence at the C-terminus of the I27 domain, pH 8.0, 37°C | Escherichia coli | |
0.053 | - |
human titin | unfolded, bearing C-terminal 20 residues of SulA sequence at the C-terminus of the I27 domain, pH 8.0, 37°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
human titin + H2O | - |
Escherichia coli | ? | - |
? | |
additional information | enzyme recognizes degrons, i.e. degradation tags. Degron tags are also regulatory elements that determine protease activity levels. Different tags fused to the same protein change degradation speeds and energetic efficiencies by 10fold or more. Degron binding to multiple sites in the Lon hexamer differentially stabilizes specific enzyme conformations, including one with high protease and low ATPase activity, and results in positively cooperative degradation | Escherichia coli | ? | - |
? |