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Literature summary for 3.4.21.53 extracted from
- Lon protease degrades transfer-messenger RNA-tagged proteins (2007), J. Bacteriol., 189, 6564-6571.
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | lon protease functions in trans translation | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| C-terminally His6-tagged lon expressed in Escherichia coli strain CH1019 carrying lon expression plasmid pET21b-LonH6 | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | in an endogenous protein tagging assay, lon mutants accumulate excessive levels of tmRNA-tagged proteins. In a reporter protein tagging assay with lambda-CI-N, lon mutants efficiently tag the reporter protein, but the tagged protein exhibits increased stability. GFP construct containing a hard-coded C-terminal tmRNA tag exhibits increased stability in lon mutant cells | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tmRNA-tagged protein + H2O | Escherichia coli | - |
? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
K-12 derivative W3110 [F- lambda- IN(rrnD-rrnE)1 rph-1] | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by successive Ni2+-NTA affinity chromatography and anion-exchange chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | lon contains three distinct domains, an amino-terminal domain having an undefined function, a central ATPase domain crucial for substrate binding and unfolding, and a C-terminal peptidase domain | Escherichia coli | ? | - |
? | |
| tmRNA-tagged protein + H2O | - |
Escherichia coli | ? | - |
? | |
| tmRNA-tagged protein + H2O | highly purified lon preferentially degrades tmRNA-tagged forms of proteins compared to untagged forms | Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lon | - |
Escherichia coli |
| lon ATP-dependent protease | - |
Escherichia coli |
| lon protease | - |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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