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Literature summary for 3.4.21.53 extracted from
- Peptidyl boronates inhibit Salmonella enterica serovar Typhimurium Lon protease by a competitive ATP-dependent mechanism (2007), Biochemistry, 46, 6647-6657.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | lon is a target in the development of antibiotics | Salmonella enterica subsp. enterica serovar Typhimurium |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant lon overexpressed in Escherichia coli BL21 (DE3), using plasmids pHF020 and pHF031, respectively | Salmonella enterica subsp. enterica serovar Typhimurium |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S680A | displays both intrinsic and peptide-stimulated ATP hydrolysis activity comparable to that of the wild-type enzyme but is unable to catalyze peptide bond hydrolysis. Active site serine is required for interaction of inhibitor with lon | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dansyl-YRGIT-Abu | not time-dependent inhibitor of peptide hydrolysis activity of lon | Salmonella enterica subsp. enterica serovar Typhimurium |  |
| dansyl-YRGIT-Abu-B(OH)2 | potent time-dependent inhibitor of peptide hydrolysis activity of lon, inhibition requires the binding but not hydrolysis of ATP, is an alternative peptidyl boronate inhibitor | Salmonella enterica subsp. enterica serovar Typhimurium | |
| MG262 | potent time-dependent inhibitor of peptide hydrolysis activity of lon, inhibition requires the binding but not hydrolysis of ATP | Salmonella enterica subsp. enterica serovar Typhimurium | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| YRGIT-Abu-SGRQK(Bz) + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lon protease | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
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