Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Brevibacillus thermoruber |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
88000 | - |
6 * 90000, SDS-PAGE, 6 * 88000, calculated | Brevibacillus thermoruber |
90000 | - |
6 * 90000, SDS-PAGE, 6 * 88000, calculated | Brevibacillus thermoruber |
549000 | - |
gel filtration | Brevibacillus thermoruber |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacillus thermoruber | Q84FG5 | expression is heat-inducible. In addition to ATPase activity and peptidase activity, enzyme has an ATP-independent chaperone-like activity | - |
Brevibacillus thermoruber WR-249 | Q84FG5 | expression is heat-inducible. In addition to ATPase activity and peptidase activity, enzyme has an ATP-independent chaperone-like activity | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
267.7 | - |
substrate succinyl-FLF-4-methoxy-beta-naphthylamide, pH 8,0, 50°C | Brevibacillus thermoruber |
697.6 | - |
substrate glutaryl-AAF-4-methoxy-beta-naphthylamide, pH 8,0, 50°C | Brevibacillus thermoruber |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutaryl-AAF-4-methoxy-beta-naphthylamide + H2O | preferred substrate | Brevibacillus thermoruber | glutaryl-L-Ala-L-Ala-L-Phe + 4-methoxy-2-naphthylamine | - |
? | |
glutaryl-AAF-4-methoxy-beta-naphthylamide + H2O | preferred substrate | Brevibacillus thermoruber WR-249 | glutaryl-L-Ala-L-Ala-L-Phe + 4-methoxy-2-naphthylamine | - |
? | |
succinyl-FLF-4-methoxy-beta-naphthylamide + H2O | - |
Brevibacillus thermoruber | succinyl-FLF + 4-methoxy-beta-naphthylamine | - |
? | |
succinyl-FLF-4-methoxy-beta-naphthylamide + H2O | - |
Brevibacillus thermoruber WR-249 | succinyl-FLF + 4-methoxy-beta-naphthylamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 90000, SDS-PAGE, 6 * 88000, calculated | Brevibacillus thermoruber |
More | protein consists of an N-terminal domain, a central ATPase domain which includes a sensor- and substrate-discrimination domain, and a C-terminal protease domain | Brevibacillus thermoruber |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
peptidase activity | Brevibacillus thermoruber |
70 | - |
ATPase activity | Brevibacillus thermoruber |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
71.5 | - |
melting temperature | Brevibacillus thermoruber |