Activating Compound | Comment | Organism | Structure |
---|---|---|---|
adenosine 5'-(3-thiotriphosphate) | i.e. adenosine 5'-O-(thiotriphosphate) or ATP-gamma-S, activation | Escherichia coli | |
adenosine 5'-(3-thiotriphosphate) | slight | Escherichia coli | |
Adenyl-5'-yl imidodiphosphate | can replace ATP | Escherichia coli | |
Adenyl-5'-yl imidodiphosphate | i.e. AMP-PNP, activation | Escherichia coli | |
adenyl-5'-yl methylene diphosphonate | i.e. AMP-PCP, activation | Escherichia coli | |
adenyl-5'-yl methylene diphosphonate | can replace ATP | Escherichia coli | |
GTP | not | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Denatured lambda Cro protein | casein as substrate | Escherichia coli | |
lambda-N-Protein | casein as substrate | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Bacteriophage lambda N-protein + H2O | Escherichia coli | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Bacteriophage lambda N-protein + H2O | - |
Escherichia coli | ? | - |
? | |
Bacteriophage lambda protein N + H2O | cleavage sites: Ala16-Gln, Ala-Glu, Ala-Lys, Leu-Asn, Leu-Glu, Ser-Lys, Cys-Ser | Escherichia coli | Hydrolyzed bacteriophage lambda protein N | - |
? | |
casein + H2O | methylcasein | Escherichia coli | hydrolyzed casein | - |
? | |
Denatured lambda Cro protein + H2O | poor substrate, inhibits casein hydrolysis | Escherichia coli | ? | - |
? | |
Glucagon + H2O | cleavage sites: Leu6-Cys(SO3H), Leu17-Val, Ala14-Leu, Val18-Cys(SO3H) | Escherichia coli | Hydrolyzed glucagon | - |
? | |
additional information | cleavage specificity | Escherichia coli | ? | - |
? | |
additional information | No substrates are lambda-repressors cI and Cro, lambda replication protein O, E. coli galactose repressor, even after heat denaturation | Escherichia coli | ? | - |
? | |
Oxidized insulin B-chain + H2O | cleavage sites | Escherichia coli | Hydrolyzed insulin B-chain | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0333 | - |
casein | - |
Escherichia coli | |
0.333 | - |
oxidized insulin B-chain | - |
Escherichia coli | |
1 | - |
bacteriophage lambda N-protein | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | requirement | Escherichia coli | |
ATP | ATP-dependent protease | Escherichia coli | |
CTP | activation | Escherichia coli | |
CTP | less efficient than ATP | Escherichia coli | |
UTP | activation | Escherichia coli | |
UTP | less efficient than ATP | Escherichia coli |