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Literature summary for 3.4.21.53 extracted from

  • Goldberg, A.L.; Menon, A.S.; Goff, S.; Chin, D.T.
    The mechanism and regulation of the ATP-dependent protease La from Escherichia coli (1987), Biochem. Soc. Trans., 15, 809-811.
    View publication on PubMed
Search for more literature for 3.4.21.53

Activating Compound

Activating Compound Comment Organism Structure
additional information no activation by ubiquitin Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Escherichia coli
Mg2+ required for maximal ATP-binding and essential for proteolytic activity Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
87000
-
 4 * 87000, Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Proteins with highly abnormal conformation + H2O Escherichia coli catalyzes inital step in the degradation of proteins with abnormal conformation as may result from nonsense or missense mutations, biosynthetic errors or intracellular denaturation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Reaction

Reaction Comment Organism Reaction ID
hydrolysis of proteins in presence of ATP hydrolysis of proteins in presence of ATP, mechanism Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Fluorogenic peptides + H2O
-
 Escherichia coli ?
-
 ?
Proteins with highly abnormal conformation + H2O catalyzes inital step in the degradation of proteins with abnormal conformation as may result from nonsense or missense mutations, biosynthetic errors or intracellular denaturation Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 87000, Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP requirement Escherichia coli
ATP enzyme tetramer has two high and two low affinity binding sites for ATP Escherichia coli
ATP two ATP molecules are hydrolyzed for each peptide bond cleaved in proteins Escherichia coli
ATP only phosphate is released after ATP hydrolysis Escherichia coli
ATP ATP-dependent protease Escherichia coli