Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Adenosine 2',3'-dialdehyde triphosphate | activation, hydrolysis at 23% the rate of ATP, supports proteolysis with 30% the efficiency of ATP | Escherichia coli | |
GTP | activation | Escherichia coli | |
GTP | hydrolysis at 113% the rate of ATP, supports proteolysis with 14% the efficiency of ATP | Escherichia coli | |
Protein substrates | activation | Escherichia coli | |
Protein substrates | stimulation of ATP hydrolysis triggers activation of the proteolytic function | Escherichia coli | |
spermidine | activation, at physiological concentration, ATP hydrolysis and protease activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine 5'-[beta,gamma-imido]triphosphate | protein and ATP hydrolysis | Escherichia coli | |
ADP | protein and ATP hydrolysis | Escherichia coli | |
beta,gamma-methylene-ATP | not | Escherichia coli | |
Dansyl fluoride | protein and ATP hydrolysis | Escherichia coli | |
diisopropyl fluorophosphate | protein and ATP hydrolysis | Escherichia coli | |
iodoacetamide | at high concentration, protein and ATP-hydrolysis | Escherichia coli | |
NaCl | at high concentrations | Escherichia coli | |
vanadate | casein and ATP hydrolysis | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
ATP | ATP hydrolysis | Escherichia coli | |
0.045 | - |
ATP | protein hydrolysis | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Escherichia coli | |
Mg2+ | 5-7 mM | Escherichia coli | |
Mg2+ | ATP hydrolysis and protease activity | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
94000 | - |
x * 94000, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | ATP-dependent serine protease | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Escherichia coli | phosphate + ADP | - |
? | |
casein + H2O | alpha-casein | Escherichia coli | hydrolyzed casein | - |
? | |
Denatured albumin + H2O | - |
Escherichia coli | ? | - |
? | |
Denatured bovine serum albumin + H2O | - |
Escherichia coli | ? | - |
? | |
Globin + H2O | - |
Escherichia coli | ? | - |
? | |
Glucagon + H2O | - |
Escherichia coli | Hydrolyzed glucagon | - |
? | |
additional information | ATP-dependent serine protease | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
multimer | x * 94000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | requirement | Escherichia coli | |
ATP | activation only in the presence of Mg2+ | Escherichia coli | |
ATP | ATP-dependent protease | Escherichia coli | |
ATP | ATP hydrolysis is essential for hydrolysis of proteins | Escherichia coli | |
CTP | activation | Escherichia coli | |
CTP | hydrolysis at 82% the rate of ATP, supports proteolysis with 31% the efficiency of ATP | Escherichia coli | |
CTP | less efficient than ATP | Escherichia coli | |
dATP | activation | Escherichia coli | |
dATP | supports proteolysis with 92% the efficiency of ATP | Escherichia coli | |
UTP | activation | Escherichia coli | |
UTP | less efficient than ATP | Escherichia coli | |
UTP | hydrolysis at 77% the rate of ATP, supports proteolysis with 22% the efficiency of ATP | Escherichia coli |