Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.53 extracted from

  • Waxman, L.; Goldberg, A.L.
    Protease La from Escherichia coli hydrolyzes ATP and proteins in a linked fashion (1982), Proc. Natl. Acad. Sci. USA, 79, 4883-4887.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
Adenosine 2',3'-dialdehyde triphosphate activation, hydrolysis at 23% the rate of ATP, supports proteolysis with 30% the efficiency of ATP Escherichia coli
GTP activation Escherichia coli
GTP hydrolysis at 113% the rate of ATP, supports proteolysis with 14% the efficiency of ATP Escherichia coli
Protein substrates activation Escherichia coli
Protein substrates stimulation of ATP hydrolysis triggers activation of the proteolytic function Escherichia coli
spermidine activation, at physiological concentration, ATP hydrolysis and protease activity Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
adenosine 5'-[beta,gamma-imido]triphosphate protein and ATP hydrolysis Escherichia coli
ADP protein and ATP hydrolysis Escherichia coli
beta,gamma-methylene-ATP not Escherichia coli
Dansyl fluoride protein and ATP hydrolysis Escherichia coli
diisopropyl fluorophosphate protein and ATP hydrolysis Escherichia coli
iodoacetamide at high concentration, protein and ATP-hydrolysis Escherichia coli
NaCl at high concentrations Escherichia coli
vanadate casein and ATP hydrolysis Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
ATP ATP hydrolysis Escherichia coli
0.045
-
ATP protein hydrolysis Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Escherichia coli
Mg2+ 5-7 mM Escherichia coli
Mg2+ ATP hydrolysis and protease activity Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
94000
-
x * 94000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli ATP-dependent serine protease ?
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
Escherichia coli phosphate + ADP
-
?
casein + H2O alpha-casein Escherichia coli hydrolyzed casein
-
?
Denatured albumin + H2O
-
Escherichia coli ?
-
?
Denatured bovine serum albumin + H2O
-
Escherichia coli ?
-
?
Globin + H2O
-
Escherichia coli ?
-
?
Glucagon + H2O
-
Escherichia coli Hydrolyzed glucagon
-
?
additional information ATP-dependent serine protease Escherichia coli ?
-
?

Subunits

Subunits Comment Organism
multimer x * 94000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP requirement Escherichia coli
ATP activation only in the presence of Mg2+ Escherichia coli
ATP ATP-dependent protease Escherichia coli
ATP ATP hydrolysis is essential for hydrolysis of proteins Escherichia coli
CTP activation Escherichia coli
CTP hydrolysis at 82% the rate of ATP, supports proteolysis with 31% the efficiency of ATP Escherichia coli
CTP less efficient than ATP Escherichia coli
dATP activation Escherichia coli
dATP supports proteolysis with 92% the efficiency of ATP Escherichia coli
UTP activation Escherichia coli
UTP less efficient than ATP Escherichia coli
UTP hydrolysis at 77% the rate of ATP, supports proteolysis with 22% the efficiency of ATP Escherichia coli