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Literature summary for 3.4.21.5 extracted from

  • Russo, G.; Gast, A.; Schlaeger, E.J.; Angiolillo, A.; Pietropaolo, C.
    Stable expression and purification of a secreted human recombinant prethrombin-2 and its activation to thrombin (1997), Protein Expr. Purif., 10, 214-225.
    View publication on PubMed
Search for more literature for 3.4.21.5

Cloned(Commentary)

Cloned (Comment) Organism
engineering of a prothrombin cDNA coding for a secreted form of prethrombin-2, production in a mammalian expression system using DXB11 cells, a mutant strain of CHO cells in which the dihydrofolate reductase gene has been deleted and an expression vector carrying the dihydrofolate reductase cDNA Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
napsagatran
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0015
-
 D-Phe-L-pipecolyl-L-Arg-4-nitroanilide recombinant thrombin Homo sapiens
0.0016
-
 D-Phe-L-pipecolyl-L-Arg-4-nitroanilide alpha thrombin Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Phe-L-pipecolyl-L-Arg-4-nitroanilide + H2O
-
 Homo sapiens D-Phe-L-pipecolyl-L-Arg + 4-nitroaniline
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
53.8
-
 D-Phe-L-pipecolyl-L-Arg-4-nitroanilide recombinant thrombin Homo sapiens
63.3
-
 D-Phe-L-pipecolyl-L-Arg-4-nitroanilide alpha thrombin Homo sapiens