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Literature summary for 3.4.21.5 extracted from

  • Petrera, N.S.; Stafford, A.R.; Leslie, B.A.; Kretz, C.A.; Fredenburgh, J.C.; Weitz, J.I.
    Long range communication between exosites 1 and 2 modulates thrombin function (2009), J. Biol. Chem., 284, 25620-25629.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.5

Inhibitors

Inhibitors Comment Organism Structure
Hirudin hirudin-(54-65) (an analog of the COOH terminus of hirudin), inhibits the exosite 2-mediated interaction of thrombin with immobilized gamma'-peptide Homo sapiens
additional information when gammaA/gammaA-fibrinogen is clotted with thrombin in the presence of HD22, gamma#-peptide (H-Val-Arg-Pro-Glu-His-Pro-Ala-Glu-Thr-Glu-Tyr(PO3H2)-Asp-Ser-Leu-Tyr(PO3H2)-Pro-Glu-Asp-Asp-Leu-OH), or prothrombin fragment 2 there is a dose-dependent and saturable decrease in thrombin binding to the resultant fibrin clots. HD22 reduces the affinity of thrombin for gammaA/gammaA-fibrin 6fold and accelerates the dissociation of thrombin from preformed gammaA/gammaA-fibrin clots Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0026
-
 D-phenylalanyl-pipecolyl-L-arginine-4-nitroanilide no additive Homo sapiens
0.0031
-
 D-phenylalanyl-pipecolyl-L-arginine-4-nitroanilide in the presence of 0.01 mM hirudin-(54-65) Homo sapiens
0.0085
-
 tosyl-Gly-Pro-Arg-4-nitroanilide in the presence of 0.01 mM HD22 Homo sapiens
0.0117
-
 tosyl-Gly-Pro-Arg-4-nitroanilide in the presence of 0.01 mM hirudin-(54-65) Homo sapiens
0.0121
-
 D-phenylalanyl-pipecolyl-L-arginine-4-nitroanilide in the presence of 0.01 mM HD22 Homo sapiens
0.0142
-
 tosyl-Gly-Pro-Arg-4-nitroanilide in the presence of 0.01 mM HD1 Homo sapiens
0.0143
-
 D-phenylalanyl-pipecolyl-L-arginine-4-nitroanilide in the presence of 0.01 mM HD1 Homo sapiens
0.0164
-
 tosyl-Gly-Pro-Arg-4-nitroanilide no additive Homo sapiens
0.1471
-
 di-L-Glu-L-Pro-L-Arg-4-nitroanilide in the presence of 0.01 mM hirudin-(54-65) Homo sapiens
0.1554
-
 di-L-Glu-L-Pro-L-Arg-4-nitroanilide in the presence of 0.01 mM HD22 Homo sapiens
0.195
-
 di-L-Glu-L-Pro-L-Arg-4-nitroanilide in the presence of 0.01 mM HD1 Homo sapiens
0.2829
-
 di-L-Glu-L-Pro-L-Arg-4-nitroanilide no additive Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
fibrinogen + H2O Homo sapiens
-
 fibrin + fibrinopeptide A + fibrinopeptide B
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-phenylalanyl-pipecolyl-L-arginine-4-nitroanilide + H2O
-
 Homo sapiens D-phenylalanyl-pipecolyl-L-arginine + 4-nitroaniline
-
 ?
di-L-Glu-L-Pro-L-Arg-4-nitroanilide + H2O
-
 Homo sapiens di-L-Glu-L-Pro-L-Arg + 4-nitroaniline
-
 ?
fibrinogen + H2O
-
 Homo sapiens fibrin + fibrinopeptide A + fibrinopeptide B
-
 ?
tosyl-Gly-Pro-Arg-4-nitroanilide + H2O
-
 Homo sapiens tosyl-Gly-Pro-Arg + 4-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
alpha-thrombin
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
101.9
-
 D-phenylalanyl-pipecolyl-L-arginine-4-nitroanilide no additive Homo sapiens
106.8
-
 D-phenylalanyl-pipecolyl-L-arginine-4-nitroanilide in the presence of 0.01 mM HD22 Homo sapiens
156.5
-
 D-phenylalanyl-pipecolyl-L-arginine-4-nitroanilide in the presence of 0.01 mM hirudin-(54-65) Homo sapiens
192.8
-
 tosyl-Gly-Pro-Arg-4-nitroanilide in the presence of 0.01 mM HD22 Homo sapiens
209.4
-
 D-phenylalanyl-pipecolyl-L-arginine-4-nitroanilide in the presence of 0.01 mM HD1 Homo sapiens
217.1
-
 di-L-Glu-L-Pro-L-Arg-4-nitroanilide in the presence of 0.01 mM HD1 Homo sapiens
231.1
-
 di-L-Glu-L-Pro-L-Arg-4-nitroanilide in the presence of 0.01 mM HD22 Homo sapiens
234.5
-
 tosyl-Gly-Pro-Arg-4-nitroanilide no additive Homo sapiens
240.3
-
 tosyl-Gly-Pro-Arg-4-nitroanilide in the presence of 0.01 mM HD1 Homo sapiens
249.5
-
 di-L-Glu-L-Pro-L-Arg-4-nitroanilide no additive Homo sapiens
251.6
-
 tosyl-Gly-Pro-Arg-4-nitroanilide in the presence of 0.01 mM hirudin-(54-65) Homo sapiens
278.6
-
 di-L-Glu-L-Pro-L-Arg-4-nitroanilide in the presence of 0.01 mM hirudin-(54-65) Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
900
-
 di-L-Glu-L-Pro-L-Arg-4-nitroanilide no additive Homo sapiens
1100
-
 di-L-Glu-L-Pro-L-Arg-4-nitroanilide in the presence of 0.01 mM HD1 Homo sapiens
1500
-
 di-L-Glu-L-Pro-L-Arg-4-nitroanilide in the presence of 0.01 mM HD22 Homo sapiens
1900
-
 di-L-Glu-L-Pro-L-Arg-4-nitroanilide in the presence of 0.01 mM hirudin-(54-65) Homo sapiens
8800
-
 D-phenylalanyl-pipecolyl-L-arginine-4-nitroanilide in the presence of 0.01 mM HD22 Homo sapiens
14300
-
 tosyl-Gly-Pro-Arg-4-nitroanilide no additive Homo sapiens
14700
-
 D-phenylalanyl-pipecolyl-L-arginine-4-nitroanilide in the presence of 0.01 mM HD1 Homo sapiens
16900
-
 tosyl-Gly-Pro-Arg-4-nitroanilide in the presence of 0.01 mM HD1 Homo sapiens
21500
-
 tosyl-Gly-Pro-Arg-4-nitroanilide in the presence of 0.01 mM hirudin-(54-65) Homo sapiens
22700
-
 tosyl-Gly-Pro-Arg-4-nitroanilide in the presence of 0.01 mM HD22 Homo sapiens
39800
-
 D-phenylalanyl-pipecolyl-L-arginine-4-nitroanilide no additive Homo sapiens
51000
-
 D-phenylalanyl-pipecolyl-L-arginine-4-nitroanilide in the presence of 0.01 mM hirudin-(54-65) Homo sapiens