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Literature summary for 3.4.21.5 extracted from

  • Centi, S.; Messina, G.; Tombelli, S.; Palchetti, I.; Mascini, M.
    Different approaches for the detection of thrombin by an electrochemical aptamer-based assay coupled to magnetic beads (2008), Biosens. Bioelectron., 23, 1602-1609.
    View publication on PubMed

Application

Application Comment Organism
analysis development of a direct and an indirect competitive assay for thrombin by an electrochemical aptamer-based assay coupled to magnetic beads. With the direct competitive assay, when the aptamer is immobilised onto the magnetic beads, a detection limit of 430 nM for thrombin is achieved. A detection limit of 175 nM is obtained by detecting the product of the enzymatic reaction catalysed by thrombin. A sandwich assay reaches a detection limit of 0.45 nM of thrombin Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
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commercial product
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-Ala-Gly-Arg-4-nitroanilide + H2O
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Homo sapiens beta-Ala-Gly-Arg + 4-nitroaniline
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