Crystallization (Comment) | Organism |
---|---|
enzyme complexed with hirugen, 5.5 mg/ml in 24-30% PEG 4000, 0.1 M sodium phosphate, pH 7.3, 20°C, vapour diffusion method, single crystals are soaked overnight in 30% PEG 4000, 0.1 M sodium phosphate, pH 7.3, and 0.5 mg/ml inhibitor, X-ray diffraction structure determination and analysis | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CRC 220 | binding mode to the enzyme, crystal structure | Homo sapiens | |
inogatran | binding mode to the enzyme, crystal structure | Homo sapiens | |
melagatran | binding mode to the enzyme, crystal structure | Homo sapiens | |
additional information | inhibition mechanism | Homo sapiens | |
napsagatran | binding mode to the enzyme, crystal structure | Bos taurus | |
napsagatran | binding mode to the enzyme, crystal structure | Homo sapiens | |
napsagatran ethyl ester | binding mode to the enzyme, crystal structure | Bos taurus | |
napsagatran ethyl ester | binding mode to the enzyme, crystal structure | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | thermodynamic analysis of enzyme structure | Homo sapiens | |
additional information | - |
additional information | thermodynamic analysis of enzyme structure | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P00735 | - |
- |
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.019 | - |
purified enzyme | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | determination of enzyme activity by active site titration with 4-nitrophenyl guanidino-benzoate | Bos taurus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-thrombin | - |
Homo sapiens |
alpha-thrombin | - |
Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics for napsagatran and napsagatran ethyl ester in presence of NaCl or KCl and at 4 different temperatures | Bos taurus |