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Literature summary for 3.4.21.5 extracted from

  • Prasad, S.; Cantwell, A.M.; Bush, L.A.; Shih, P.; Xu, H.; Di Cera, E.
    Residue Asp-189 controls both substrate binding and the monovalent cation specificity of thrombin (2004), J. Biol. Chem., 279, 10103-10108.
    View publication on PubMed
Search for more literature for 3.4.21.5

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in baby hamster kidney cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
D189A site-directed mutagenesis, reduced substrate and monovalent cation specificity and enzyme activity Homo sapiens
D189E site-directed mutagenesis, reduced substrate and monovalent cation specificity and enzyme activity Homo sapiens
D189N site-directed mutagenesis, reduced substrate and monovalent cation specificity and enzyme activity Homo sapiens
D189S site-directed mutagenesis, reduced substrate and monovalent cation specificity and proteolytic activity, amidolytic activity is slightly reduced Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
antithrombin
-
 Homo sapiens
Hirudin
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activates, can partially substitute for Na+, binding to Asp189 Homo sapiens
Li+ activates, can partially substitute for Na+, binding to Asp189 Homo sapiens
additional information mutants show reduced specificity for monovalent cations compared to the wild-type enzyme Homo sapiens
Na+ activates, binding to Asp189, most efficient monovalent cation Homo sapiens
Rb+ activates, can partially substitute for Na+, binding to Asp189 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification activation of the recombinant wild-type and mutant enzymes by snake venom enzyme ecarin Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes to homogeneity Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
selective cleavage of Arg-/-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B Asp189 is involved in substrate recognition Homo sapiens
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-Pro-Phe-4-nitroanilide + H2O synthetic chromogenic substrate Homo sapiens Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?
D-Phe-Pro-Arg-4-nitroanilide + H2O synthetic chromogenic substrate Homo sapiens D-Phe-Pro-Arg + 4-nitroaniline
-
 ?
D-Phe-Pro-Lys-4-nitroanilide + H2O synthetic chromogenic substrate Homo sapiens D-Phe-Pro-Lys + 4-nitroaniline
-
 ?
fibrinogen + H2O proteolytic activation Homo sapiens fibrin + fibrinopeptide A + fibrinopeptide B
-
 ?
PAR1 + H2O protease-activated receptor 1 Homo sapiens ?
-
 ?
PAR3 + H2O protease-activated receptor 3 Homo sapiens ?
-
 ?
PAR4 + H2O protease-activated receptor 4 Homo sapiens ?
-
 ?
protein C + H2O
-
 Homo sapiens ?
-
 ?