Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme immobilized on Sepharose has higher thermal stability than the wild-type enzyme, enzyme immobilized on porous glass loaded with isothiocyanate propyl groups has the same thermal stability as the wild-type enzyme, enzyme immobilized on DEAE-Sephadex has lower thermal stability than the wild-type enzyme | Cambarus affinis |
General Stability | Organism |
---|---|
enzyme immobilized on Sepharose has higher thermal stability than the wild-type enzyme, enzyme immobilized on porous glass loaded with isothiocyanate propyl groups has the same thermal stability as the wild-type enzyme, enzyme immobilized on DEAE-Sephadex has lower thermal stability than the wild-type enzyme | Cambarus affinis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
tosyl-Arg methyl ester | free enzyme | Cambarus affinis | |
1.53 | - |
tosyl-Arg methyl ester | enzyme immobilized on Sepharose 4B-KT | Cambarus affinis | |
2.5 | - |
tosyl-Arg methyl ester | enzyme immobilized on porous glass loaded with isothiocyanate | Cambarus affinis | |
6.2 | - |
tosyl-Arg methyl ester | enzyme immobilized on Sepharose 4B-KT | Cambarus affinis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cambarus affinis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-tosyl-L-Arg methyl ester + H2O | - |
Cambarus affinis | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
hydrolysis of tosyl-Arg methyl ester, enzyme immobilized on DEAE-Sephadex A-50 KT | Cambarus affinis |
8.5 | - |
hydrolysis of tosyl-Arg methyl ester, free enzyme | Cambarus affinis |
9 | - |
hydrolysis of tosyl-Arg methyl ester, enzyme immobilized on porous glass with isothiocyanate propyl groups | Cambarus affinis |
9.5 | - |
hydrolysis of tosyl-Arg methyl ester, enzyme immobilized on Sepharose 4B-KT | Cambarus affinis |