Protein Variants | Comment | Organism |
---|---|---|
K188F | increase of Km for Arg and Lys containing substrates, cleavage of nearly 30 new peptide bonds in beta-casein compared to the wild-type enzyme | Rattus norvegicus |
K188W | increase of Km for Arg and Lys containing substrates, 3-4fold decrease in turnover number | Rattus norvegicus |
K188Y | increase of Km for Arg and Lys containing substrates, cleavage of nearly 30 new peptide bonds in beta-casein compared to the wild-type enzyme | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | influence of pH-value on Km-value of wild-type and mutant enzymes | Rattus norvegicus | |
0.033 | - |
succinyl-Ala-Ala-Pro-Arg-p-nitroanilide | wild-type enzyme, pH 8 | Rattus norvegicus | |
0.057 | - |
succinyl-Ala-Ala-Pro-Arg-p-nitroanilide | wild-type enzyme, pH 9 | Rattus norvegicus | |
0.087 | - |
succinyl-Ala-Ala-Pro-Arg-p-nitroanilide | wild-type enzyme, pH 7 | Rattus norvegicus | |
0.099 | - |
succinyl-Ala-Ala-Pro-Arg-p-nitroanilide | wild-type enzyme, pH 10 | Rattus norvegicus | |
0.101 | - |
succinyl-Ala-Ala-Pro-Lys-p-nitroanilide | wild-type enzyme, pH 9 | Rattus norvegicus | |
0.105 | - |
succinyl-Ala-Ala-Pro-Lys-p-nitroanilide | wild-type enzyme, pH 8 | Rattus norvegicus | |
0.184 | - |
succinyl-Ala-Ala-Pro-Lys-p-nitroanilide | wild-type enzyme, pH 10 | Rattus norvegicus | |
0.839 | - |
succinyl-Ala-Ala-Pro-Lys-p-nitroanilide | wild-type enzyme, pH 7 | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | beta-casein, mutant enzymes K188E and K188Y cleave nearly 30 new peptide bonds compared to wild-type enzyme | Rattus norvegicus | ? | - |
? | |
succinyl-Ala-Ala-Pro-Arg-p-nitroanilide + H2O | - |
Rattus norvegicus | ? | - |
? | |
succinyl-Ala-Ala-Pro-Lys-p-nitroanilide + H2O | - |
Rattus norvegicus | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | influence of pH-value on turnover number of wild-type and mutant enzymes | Rattus norvegicus | |
40 | - |
succinyl-Ala-Ala-Pro-Lys-p-nitroanilide | wild-type enzyme, pH 7 | Rattus norvegicus | |
59 | - |
succinyl-Ala-Ala-Pro-Lys-p-nitroanilide | wild-type enzyme, pH 9 | Rattus norvegicus | |
64 | - |
succinyl-Ala-Ala-Pro-Lys-p-nitroanilide | wild-type enzyme, pH 10 | Rattus norvegicus | |
74 | - |
succinyl-Ala-Ala-Pro-Lys-p-nitroanilide | wild-type enzyme, pH 8 | Rattus norvegicus | |
95 | - |
succinyl-Ala-Ala-Pro-Arg-p-nitroanilide | wild-type enzyme, pH 7 | Rattus norvegicus | |
144 | - |
succinyl-Ala-Ala-Pro-Arg-p-nitroanilide | wild-type enzyme, pH 9 | Rattus norvegicus | |
145 | - |
succinyl-Ala-Ala-Pro-Arg-p-nitroanilide | wild-type enzyme, pH 10 | Rattus norvegicus | |
163 | - |
succinyl-Ala-Ala-Pro-Arg-p-nitroanilide | wild-type enzyme, pH 8 | Rattus norvegicus |