General Stability | Organism |
---|---|
no loss of activity upon modification up to 75-85% with monomeric glutaraldehyde, polymeric glutaraldehyde, oxidized sucrose and oxidized sucrose polymers. Modified trypsin resists exposure to 8 M urea in the presence and absence of 5 mM 2-mercaptoethanol, native enzyme loses its activity in 20 and 120 min | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
bovine serum albumin | - |
Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Bovine serum albumin + H2O | - |
Bos taurus | ? | - |
? | |
N-Benzoyl-L-Arg ethyl ester + H2O | - |
Bos taurus | ? | - |
? | |
Nalpha-benzoyl-DL-Arg-p-nitroanilide + H2O | - |
Bos taurus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
native enzyme | Bos taurus |
55 | - |
enzyme modified with sucrose | Bos taurus |
60 | - |
enzyme modified with monomeric glutaraldehyde | Bos taurus |
68 | - |
enzyme modified with polymeric glutaraldehyde | Bos taurus |
74 | 76 | enzyme modified with oxidized sucrose polymers | Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
half-life of native enzyme: 29.4 h | Bos taurus |
80 | - |
half-life of native enzyme: 126 s | Bos taurus |