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Literature summary for 3.4.21.4 extracted from

  • Jeohn, G.H.; Serizawa, S.; Iwamatsu, A.; Takahashi, K.
    Isolation and characterization of gastric trypsin from the microsomal fraction of porcine gastric antral mucosa (1995), J. Biol. Chem., 270, 14748-14755.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Sus scrofa
benzamidine
-
Sus scrofa
chymostatin
-
Sus scrofa
DFP
-
Sus scrofa
EDTA
-
Sus scrofa
Hg2+
-
Sus scrofa
leupeptin
-
Sus scrofa
Pancreatic trypsin inhibitor bovine pancreatic trypsin inhibitor Sus scrofa
Zn2+
-
Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Sus scrofa
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22400
-
x * 22400, SDS-PAGE under nonreducing conditions Sus scrofa
28000
-
x * 28000, SDS-PAGE under reducing conditions Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
enzyme form A and B Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
gastric mucosa antral mucosa Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.897
-
form B Sus scrofa
0.9397
-
form A Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tert-butyloxycarbonyl-Gln-Ala-Arg-4-methylcoumarin 7-amide + H2O
-
Sus scrofa ?
-
?
tert-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumarin 7-amide + H2O
-
Sus scrofa ?
-
?
tert-butyloxycarbonyl-Gln-Gly-Arg-4-methylcoumarin 7-amide + H2O
-
Sus scrofa ?
-
?

Subunits

Subunits Comment Organism
? x * 22400, SDS-PAGE under nonreducing conditions Sus scrofa
? x * 28000, SDS-PAGE under reducing conditions Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
Sus scrofa