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Literature summary for 3.4.21.4 extracted from
- Enzymic and physicochemical properties of Streptomyces griseus trypsin (1975), Biochemistry, 14, 1161-1167.
General Stability
| General Stability | Organism |
|---|---|
| significant stabilization in the neutral and alkaline range by 15 mM Ca2+ | Streptomyces griseus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-1-chloro-3-tosylamido-4-phenyl-2-butanone | - |
Streptomyces griseus |  |
| p-tosyl-L-Lys-chloromethane | reaction towards B chain of insulin is inhibited, the activity towards the A-chain is not significantly affected | Streptomyces griseus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0077 | - |
N-benzoyl-L-Arg ethyl ester | - |
Streptomyces griseus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22800 | - |
low-speed equilibrium sedimentation | Streptomyces griseus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces griseus | - |
- |
- |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| reversible denaturation at pH 12.0 and at pH 2.0 | Streptomyces griseus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, pH 2-10, stable | Streptomyces griseus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| insulin + H2O | oxidized A chain, and B chain | Streptomyces griseus | ? | - |
? | |
| N-Benzoyl-L-Arg ethyl ester + H2O | - |
Streptomyces griseus | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
maximal stability at pH 3-4 | Streptomyces griseus |
| 30 | - |
pH 8.0, 75% loss of activity after 25 min, 90% loss of activity after 24 h | Streptomyces griseus |
| 30 | - |
pH 10.0, complete loss of activitry after 25 min | Streptomyces griseus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
significant stabilization in the neutral and alkaline range by 15 mM Ca2+ | Streptomyces griseus |
| 1 | - |
0°C, 12 h, 20% loss of activity | Streptomyces griseus |
| 2 | 10 | 0°C, very stable | Streptomyces griseus |
| 10 | - |
20% loss of activity after 12 h at 0°C, complete loss of activity after 25 min at 30°C | Streptomyces griseus |
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