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Literature summary for 3.4.21.4 extracted from

  • Valdez-Melchor, R.; Ezquerra-Brauer, J.; Cinco-Moroyoqui, F.; Castillo-Yanez, F.; Cardenas-Lopez, J.
    Purification and partial characterization of trypsin from the viscera of tropical sierra (Scomberomorus Sierra) from the Gulf of California (2013), J. Food Biochem., 37, 694-701.
No PubMed abstract available
Search for more literature for 3.4.21.4

Inhibitors

Inhibitors Comment Organism Structure
porcine trypsin inhibitor 0.01 mM, more than 95% inhibition Scomberomorus sierra
Soybean trypsin inhibitor 0.01 mM, more than 95% inhibition Scomberomorus sierra

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25400
-
 x * 25400, SDS-PAGE Scomberomorus sierra

Organism

Organism UniProt Comment Textmining
Scomberomorus sierra
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Scomberomorus sierra

Source Tissue

Source Tissue Comment Organism Textmining
viscus
-
 Scomberomorus sierra
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-alpha-benzoyl-DL-Arg-4-nitroanilide + H2O
-
 Scomberomorus sierra N-alpha-benzoyl-DL-Arg + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 25400, SDS-PAGE Scomberomorus sierra

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Scomberomorus sierra

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 30 min, 40% loss of activity Scomberomorus sierra
60
-
 30 min, 80% loss of activity Scomberomorus sierra

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Scomberomorus sierra

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 10 enzyme maintains more than 50% of its activity for a period of 2 h of incubation in the pH range 8-10 Scomberomorus sierra