Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzamidine | 5 mM, 17.9% residual activity | Channa argus | |
E-64 | 0.02 mM, 19.5% residual activity | Channa argus | |
Lima bean trypsin inhibitor | 0.002 mM, 7.9% residual activity | Channa argus | |
mung bean trypsin inhibitor | 0.003 mM, 3.6% residual activity | Channa argus | |
Pefabloc SC | 1 mM, 4.5% residual activity | Channa argus | |
phenylmethylsulfonyl fluoride | 10 mM, 4.5% residual activity | Channa argus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00049 | - |
benzyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin | pH 8.0, 37°C | Channa argus | |
0.00059 | - |
benzyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin | pH 8.0, 37°C | Channa argus | |
0.00064 | - |
benzyloxycarbonyl-Glu-Arg-Arg-7-amido-4-methylcoumarin | pH 8.0, 37°C | Channa argus | |
0.00092 | - |
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin | pH 8.0, 37°C | Channa argus | |
0.00102 | - |
benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin | pH 8.0, 37°C | Channa argus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, 114% of initial activity | Channa argus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
x * 22000, SDS-PAGE | Channa argus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Channa argus | G3LUK8 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | sequence contains a signal peptide of 15 residues and an activation peptide of 9 amino acid residues with a mature protein of 223 residues | Channa argus |
Purification (Comment) | Organism |
---|---|
- |
Channa argus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hepatopancreas | - |
Channa argus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
502 | - |
pH 8.0, 37°C | Channa argus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin + H2O | 94.8% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin | Channa argus | benzyloxycarbonyl-Gln-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Glu-Arg-Arg-7-amido-4-methylcoumarin + H2O | 75.2% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin | Channa argus | benzyloxycarbonyl-Glu-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin + H2O | 14.8% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin | Channa argus | benzyloxycarbonyl-Glu-Lys-Lys + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin + H2O | 51.4% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin | Channa argus | benzyloxycarbonyl-Leu-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin + H2O | 44.5% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin | Channa argus | benzyloxycarbonyl-Leu-Lys-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O | - |
Channa argus | benzyloxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O | 27.1% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin | Channa argus | benzyloxycarbonyl-Val-Leu-Lys + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin + H2O | 33.5% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin | Channa argus | benzyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin | - |
? | |
additional information | no substrates: benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin, benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin, succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin, Lys-7-amido-4-methylcoumarin and Arg-7-amido-4-methylcoumarin | Channa argus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 22000, SDS-PAGE | Channa argus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Channa argus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
stable below | Channa argus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
72 | - |
benzyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin | pH 8.0, 37°C | Channa argus | |
76 | - |
benzyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin | pH 8.0, 37°C | Channa argus | |
80 | - |
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin | pH 8.0, 37°C | Channa argus | |
92 | - |
benzyloxycarbonyl-Glu-Arg-Arg-7-amido-4-methylcoumarin | pH 8.0, 37°C | Channa argus | |
148 | - |
benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin | pH 8.0, 37°C | Channa argus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Channa argus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | - |
Channa argus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
87200 | - |
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin | pH 8.0, 37°C | Channa argus | |
122200 | - |
benzyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin | pH 8.0, 37°C | Channa argus | |
143300 | - |
benzyloxycarbonyl-Glu-Arg-Arg-7-amido-4-methylcoumarin | pH 8.0, 37°C | Channa argus | |
144700 | - |
benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin | pH 8.0, 37°C | Channa argus | |
153900 | - |
benzyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin | pH 8.0, 37°C | Channa argus |