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Literature summary for 3.4.21.4 extracted from

  • Gardner, Q.A.; Younas, H.; Akhtar, M.
    Studies on the regioselectivity and kinetics of the action of trypsin on proinsulin and its derivatives using mass spectrometry (2013), Biochim. Biophys. Acta, 1834, 182-190.
    View publication on PubMed
Search for more literature for 3.4.21.4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0085
-
 M-diarginyl insulin pH 8.0, 37°C Bos taurus
0.0092
-
 GERGFFYTPKTRR pH 8.0, 37°C Bos taurus
0.0153
-
 Nalpha-benzoyl-L-arginine ethyl ester pH 8.0, 37°C Bos taurus
0.1115
-
 GERGFFYTPKT pH 8.0, 37°C Bos taurus
0.115
-
 human M-proinsulin pH 8.0, 37°C Bos taurus
0.12
-
 Insulin pH 8.0, 37°C Bos taurus
0.12
-
 M-insulin pH 8.0, 37°C Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Bos taurus
-
pancreas
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bovine M-proinsulin + H2O insulin containing methionine at the N-terminus Bos taurus ? cleaved at the K59R60-G61 bond of the C/A junction, but at the B/C junction cleavage occurs at the R31R32-E33 as well as the R31-R32E33 bond. Cleavage at the B/C junction is preferred (65%) over that at the C/A junction (35%) ?
GERGFFYTPKT + H2O insulin-derived peptide Bos taurus ? cleavage at bond K-T ?
GERGFFYTPKTRR + H2O insulin-derived peptide Bos taurus ? cleavage at bond K-T ?
human M-proinsulin + H2O insulin containing methionine at the N-terminus Bos taurus ? cleavage at the R31R32-E33 and K64R65-G66 bonds, i.e. B/C and C/A junctions. Cleavage at the B/C junction is preferred (65%) over that at the C/A junction (35%) ?
insulin
-
 Bos taurus ? cleavage at bond K29-T30 ?
M-diarginyl insulin + H2O insulin containing methionine at the N-terminus Bos taurus ? cleavage at bond K29-T30 ?
M-insulin insulin containing methionine at the N-terminus Bos taurus ? cleavage at bond K29-T30 ?
Nalpha-benzoyl-L-arginine ethyl ester + H2O
-
 Bos taurus Nalpha-benzoyl-L-arginine + ethanol
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.14
-
 Insulin pH 8.0, 37°C Bos taurus
0.14
-
 M-insulin pH 8.0, 37°C Bos taurus
0.313
-
 GERGFFYTPKT pH 8.0, 37°C Bos taurus
1.1
-
 M-diarginyl insulin pH 8.0, 37°C Bos taurus
4.15
-
 GERGFFYTPKTRR pH 8.0, 37°C Bos taurus
11.65
-
 human M-proinsulin pH 8.0, 37°C Bos taurus
16.5
-
 Nalpha-benzoyl-L-arginine ethyl ester pH 8.0, 37°C Bos taurus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.27
-
 Insulin pH 8.0, 37°C Bos taurus
3.2
-
 GERGFFYTPKT pH 8.0, 37°C Bos taurus
102
-
 human M-proinsulin pH 8.0, 37°C Bos taurus
130
-
 M-diarginyl insulin pH 8.0, 37°C Bos taurus
130
-
 M-insulin pH 8.0, 37°C Bos taurus
450
-
 GERGFFYTPKTRR pH 8.0, 37°C Bos taurus
1100
-
 Nalpha-benzoyl-L-arginine ethyl ester pH 8.0, 37°C Bos taurus