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Literature summary for 3.4.21.4 extracted from

  • Pereira, H.J.; Salgado, M.C.; Oliveira, E.B.
    Immobilized analogues of sunflower trypsin inhibitor-1 constitute a versatile group of affinity sorbents for selective isolation of serine proteases (2009), J. Chromatogr. B, 877, 2039-2044.
    View publication on PubMed
Search for more literature for 3.4.21.4

Inhibitors

Inhibitors Comment Organism Structure
sunflower trypsin inhibitor-1 SFTI-1, a natural 14-residue cyclic peptide, can be used for enzyme separation from other serine proteases, synthesis of SFTI-1-based supports and affinity chromatography, overview. Inhibition of trypsin by some acyclic SFTI-1 analogues, e.g. [Lys5]-SFTI-1, [Arg5]-SFTI-1, and [Phe5]-SFTI-1, no inhibition by [Leu5]-SFTI-1, overview Bos taurus
[Arg5]-SFTI-1 a wild-type SFTI-1 analogue Bos taurus
[Lys5]-SFTI-1 a wild-type SFTI-1 analogue Bos taurus
[Phe5]-SFTI-1 a wild-type SFTI-1 analogue Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
separation of serine proteases from a mixture by sunflower trypsin inhibitor-1 affinity chromatography Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-benzoyl-DL-arginine 4-nitroanilide + H2O
-
 Bos taurus N-benzoyl-DL-arginine + 4-nitroaniline
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.1
-
 assay at Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000001
-
 [Lys5]-SFTI-1 pH 8.1, 37°C Bos taurus
0.00001
-
 [Arg5]-SFTI-1 pH 8.1, 37°C Bos taurus
0.00022
-
 [Phe5]-SFTI-1 pH 8.1, 37°C Bos taurus